The binding of peptides to MHC class I molecules induces MHC/peptide complexes that have specific conformational features. Little is known about the molecular and structural bases required for an optimal MHC/peptide association able to induce a dominant T cell response. We sought to characterize the interaction between purified HLA-A3 molecules and four well known CD8 epitopes from HIV-1 protei...

BACKGROUND We have investigated serum levels of immunoreactive marinobufagenin (MBG) in 16- to 20-week-old spontaneously hypertensive rats (SHRs)-A3 and in the normotensive Wistar-Kyoto (WKY) rat strain in the absence of salt loading, and we have investigated the genetic control of serum MBG. METHODS AND RESULTS We genotyped the F2 progeny of an SHR-A3×WKY intercross using a genome-wide panel...

The parasite Trypanosoma brucei, the causative agent of sleeping sickness across sub-Saharan Africa, depends on a remarkable U-insertion/deletion RNA editing process in its mitochondrion. A approximately 20 S multi-protein complex, called the editosome, is an essential machinery for editing pre-mRNA molecules encoding the majority of mitochondrial proteins. Editosomes contain a common core of t...

This paper presents the design and implementation of Application-Aware Anonymity (A3), an extensible platform for rapidly prototyping and evaluating anonymity protocols on the Internet. A3 supports the development of highly tunable anonymous protocols that enable applications to tailor their anonymity properties and performance characteristics according to specific communication requirements. T...

This paper has no novel learning or statistics: it is concerned with making a wide class of preexisting statistics and learning algorithms computationally tractable when faced with data sets with massive numbers of records or attributes. It briefly reviews the static AD-tree structure of Moore and Lee (1998), and offers a new structure with more attractive properties: (1) the new structure scal...

The role of adenosine A1 and A3 receptors in mediating cardioprotection has been studied predominantly in rabbits, yet the pharmacological characteristics of rabbit adenosine A1 and A3 receptor subtypes are unknown. Thus, the rabbit adenosine A3 receptor was cloned and expressed, and its pharmacology was compared with that of cloned adenosine A1 receptors. Stable transfection of rabbit A1 or A3...

Chronic exposure to nicotine has been reported to increase the number of nicotinic acetylcholine receptors (AChRs) in brain. The mechanism of up-regulation for the a4b2 AChR subtype, which accounts for the majority of high affinity nicotine binding in mammalian brain, has previously been shown to involve a decrease in the rate of a4b2 AChR turnover. Here, we report an investigation of the exten...

BACKGROUND Available study revealed advanced tumors have a higher expression rate of MAGE-A3 gene which has a lot of single nucleotide polymorphism (SNP) loci with polymorphisms. This study aimed to analyze the allele frequency of SNP loci in MAGE-A3 gene and investigate the relationship between MAGE-A3 gene polymorphisms and clinical factors. METHODS Tumor samples of a cohort of 191 NSCLC pa...