Chronic myeloid leukemia (CML) originates in the hematopoietic stem cell because of a reciprocal translocation of chromosomes 9 and 22. This translocation juxtaposes the ABL1 proto-oncogene with BCR, which translates into a constitutively active BCR-ABL kinase that drives expansion of leukemic progeny. Treatment of CML with ABL kinase inhibitors such as imatinib is very effective and brings abo...

Previous studies have shown that BCR/ABL oncogene, the molecular counterpart of the Ph1 chromosome, could represent a privileged target to natural killer (NK) cells. In the present study, we showed that activated peripheral NK cells killed high-level BCR/ABL transfectant UT-7/9 derived from the pluripotent hematopoietic cell line UT-7 with a high efficiency. To further define the mechanisms con...

More than 95% of patients with detected translocation t(9;22), is characterized by the fusion between exons e13 or e14 of BCR gene, which are located in major breakpoint cluster region (M-bcr) and exon a2 of ABL gene. These fusions are described as b2a2 (e13a2) and b3a2 (e14a2). Other fusions of exons e1, e6, e8, e12, e19, e20 of BCR gene with exons a2 or a3 of ABL gene occur very rarely and le...

Chronic myeloid leukaemia (CML) is a hematopoietic stem cell (HSC) disorder accounting for about 15-20% of all leukemias of the adult (Goldman & Melo, 2003; Black et al., 1997). The main haematological features are represented by an increase in the number of circulating mature granulocytes and their precursors and, subsequently, by a secondary evolution in acute leukaemia. In 1960, a major clue...

...........................................................................................................................7 Introduction .....................................................................................................................9 Review of the literature ..................................................................................................10 1. History of ...

BACKGROUND/AIMS New molecular cytogenetic techniques are increasingly applied as a routine investigative tool in haematological malignancies, both at diagnosis and subsequent monitoring. This report describes the interpretation of atypical signal patterns encountered using BCR-ABL dual colour dual fusion fluorescence in situ hybridisation (D-FISH) translocation probes in chronic myeloid leukaem...

The (9;22) translocation which produces the Philadelphia (Ph1) chro mosome activates the ahi oncogene from chromosome 9 by recombination with the her gene from chromosome 22. This fusion gene is transcribed into a new 8.5-kilobase chimeric niRN A which is translated into a novel M, 210,000 fusion protein which has a protein tyrosine kinase activity that is greatly increased in comparison to the...

Background. FISH is a molecular cytogenetic technique enabling rapid detection of genetic abnormalities. Facilities that can run fresh/wet samples for molecular diagnosis and monitoring of neoplastic disorders are not readily available in Ghana and other neighbouring countries. This study aims to demonstrate that interphase FISH can successfully be applied to archival methanol-fixed bone marrow...

Chronic myeloid leukaemia (CML) is a clonal myeloproliferative disorder arising as a result of the reciprocal translocation between the long arms of chromosomes 9 and 22 (t9;22), leading to the formation of the fusion oncogene BCR-ABL. BCR-ABL has constitutive tyrosine kinase (TK) activity which is necessary for the transformed phenotype. The introduction at the end of the last century of BCR-A...