The postmicrosomal protein fraction obtained from distal stumps of rat sciatic nerves at 0-6 days following transection were investigated by means of one- and two-dimensional electrophoresis. In all experimental groups, total amount of protein was significantly higher than in the control group. Proteins were resolved into 27 bands after SDS-PAGE. Their molecular weights ranged between 16.2 and ...

CO2 emissions are the primary reason for global warming; hence, biological and chemical technologies converting into useful compounds being actively studied. Biological methods using enzymes can convert under mild conditions. Formate dehydrogenase (FDH) is a representative conversion enzyme. Its function was revealed after isolation from bacteria, yeast, plants. In this study, we evaluated pote...

The habitats of Batillaria multiformis (Near Threatened species) and attramentaria are often overlapping on a mudflat, young individuals two snails were difficult to identify by shell morphology. This is one the causes that study, such as population dynamics species, did not advance. Recently, species identification DNA analysis for B. was developed using PCR-RFLP method. However, only restrict...

Proteins labeled with a luminescent lanthanide chelate, {{2,2',2'',2'''-{4'-{[(4,6-dichloro-1,3,5-triazin-2-yl)amino]biphenyl-4-yl}-2,2':6',2''-terpyridine-6,6''-diyl}bis(methylenenitrilo)}tetrakis(acetato)}europium(III) (DTBTA-Eu(3+)),were analyzed with sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) slab gel electrophoresis and hydroxyethyl cellurose gel capillary electrophoresis with a ...

A new fast and reliable reversed-phase high-performance liquid chromatographic method is proposed for quantifying all the major casein fractions during cheese proteolysis as an alternative to more complex methodologies. Excellent separation of caseins, para-κ-casein (ρ-κ-CN), αs1-casein (αs1-CN), αs1-I-casein (αs1-I-CN), αs2-casein (αs2-CN), β-casein (β-CN) was achieved, in terms linearity, rep...

This case presents haemoglobin D Trait (Hb-D) which is a rare disease in Bangladesh. It heterozygous state for who came to Laboratory Medicine Department of Chattogram Maa Shishu-O-General Hospital (CMSOGH) heamoglobin electrophoresis. Our patient was 9 years old girl from Noakhali, She clinically silent with no splenomegaly. Her peripheral blood film microcytic hypochromic anaemia and Capillar...

References 1. Thiede C, Florek M, Bornhauser M, Ritter M, Mohr B, Brendel C, et al. Rapid quantification of mixed chimerism using multiplex amplification of short tandem repeat markers and fluorescence detection. Bone Marrow Transplant 1999;23:1055–60. 2. Lawler M, Humphries P, McCann SR. Evaluation of mixed chimerism by in vitro amplification of dinucleotide repeat sequences using the polymera...

Stereomicroscope The new Carl Zeiss stereomicroscope Stemi 2000-CS allows the user to view the sample whilst other observers view the procedure by video. Greenough type stereomicroscopes create their stereo effect by having separate light paths set at different angles, one for each eye. However, when video or photomicrography is being carried out most stereomicroscope systems remove light from ...

A patient with pancreatic ascites is described. Electrophoresis of the patient's serum demonstrated bisalbuminaemia, which disappeared after resolution of the ascites.