Background: Dengue is an acute viral infection has emerged as a notable public health problem. Rapid immunochromatographic test and IgM/IgG ELISA been the mainstay of diagnosis. Combination NS1 antigen detection along with antibody increases diagnostic rates. Thrombocytopenia begins during febrile phase. Therefore, we tried to evaluate association platelet counts against (NS1 IgM/IgG) IgM in de...

Sensitivities of two enzyme-linked immunosorbent assays (ELISAs) with particulate and sodium dodecyl sulfate (SDS)-disrupted Anaplasma marginale antigen were compared. The quotient of positive reference sera divided by the absorbance quotient of a negative reference serum at identical dilution was termed the signal-to-noise ratio. Optimal signal-to-noise ratios were dependent on both pretreatme...

Background: Hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of Echinococcus granulosus. Diagnosis of hydatidosis is still an unresolved problem. Serological tests using crude antigens for diagnosis of E. granulosus are sensi-tive, however their specificity are not satisfactory. Therefore, WHO recommended spe-cific serological methods using specific antigens,...

background: cystic echinococcosis (ce), also known as echinococcosis/hydatidosis, is one of the most important parasitic diseases in the world. it enhances both humoral and cellular (th1 and th2) responses in infected host. different antigens of the worm may favor the th1 or th2 immune responses in ce patients. objective: to evaluate the humoral and cellular immune responses of balb/c mice agai...

Real-time PCR is a very sensitive technique to measure DNA concentrations. In real-time immuno-PCR, it is used as the detection system for quantification of proteins. Many ways to perform and assemble real-time immuno-PCR are possible. We have tested three different approaches for the detection of prostate specific antigen (PSA) and compared them with each other and with ELISA. We also demonstr...

The clinical usefulness of antineutrophil cytoplasmic antibodies (ANCAs) in the monitoring of patients treated for small vessel vasculitis is debated. A capture enzyme-linked immunosorbent assay (ELISA) based on anti-proteinase 3 (anti-PR3) monoclonal antibody 4A3 has previously been proven to be superior to indirect immunofluorescence (IIF) and standard ELISA for the diagnosis of vasculitis. T...