A new liquid culture medium prepared with chemicals that can be obtained economically and commercially was tested in in vitro cultivation of Leishmania promastigotes to obtain a large number of organisms to use in serological studies. The number of Leishmania infantum and Leishmania tropica promastigotes taken from Novy-MacNeal-Nicolle (NNN) medium reached 1 x 10(7)/ml at the end of the 8th day...

A estrutura dos genes ribossômicos de Leishmania foi caracterizada, através da determina­ ção de seus padrões de restrição, com o objetivo de descrever regiões do gene que permitam a identifica­ ção destes organismos. A análise comparativa dos padrões de restrição de seis espécies de Leishmania permitiu identificar heterogeneidade intra e interespecífica na organização das regiões espaçadoras d...

P2P-based Application Layer Multicast (ALM) systems have shown a great success for several group communication applications. But some performance problems still await a major breakthrough from these systems for critical services such as live video streaming. For these applications, one of the problems is the dynamics of users’ presence since the unannounced departure of a peer causes an interru...

cutaneous leishmaniasis is one of the most important parasitic diseases, which are endemic in different parts of iran.   leishmania major and l. tropica are the primary causative agents of this disease. the aim of the present study was to detect the multiple forms of l. major in lung. ppromastigotes of l. major at stationary phase were injected to balb/c mice. after 60 days, the different forms...

Recently, the accessory limb model (ALM) has become an alternative study system for limb regeneration studies in axolotls instead of using an amputated limb. ALM progresses limb regeneration study in axolotls because of its advantages. To apply and/or to compare knowledge in axolotl ALM studies to other vertebrates is a conceivable next step. First, Xenopus laevis, an anuran amphibian, was inve...

We study fully hydrated bilayers of two di-monounsaturated phospholipids diC18:1PC (DOPC) and diC22:1PC with varying amounts of alamethicin (Alm). We combine the use of X-ray diffuse scattering and molecular dynamics simulations to determine the orientation of alamethicin in model lipids. Comparison of the experimental and simulated form factors shows that Alm helices are inserted transmembrane...

Background: Cationic immune stimulating complexes (PLUSCOMs) are particulate antigen delivery systems. PLUSCOMs consist of cationic immunostimulatory complexes (ISCOMs) derivatives and are able to elicit in vivo T cell responses against an antigen. Objective: To evaluate the effects of PLUSCOMs containing Leishmaniamajor antigens (SLA) on the type of immune response generated in the murine mod...

Infection of dendritic cells by the human protozoal parasite Leishmania is part of its survival strategy. The dendritic cell receptors for Leishmania have not been established and might differ in their interactions among Leishmania species and infective stages. We present evidence that the surface C-type lectin DC-SIGN (CD 209) is a receptor for promastigote and amastigote infective stages from...

background pentavalent antimonials remain the choice of treatment for leishmaniasis, despite their toxicity, high cost, and difficult administration. as an alternative, morphine may induce the healing process of cutaneous leishmaniasis by its immunoregulatory characteristics. objectives to study the effect of morphine on the wound-healing process of cutaneous leishmaniasis (cl) in a mouse model...