نتایج جستجو برای: bacterial reverse mutation assay
تعداد نتایج: 777536 فیلتر نتایج به سال:
Background: JAK2 is a nonreceptor tyrosine kinase that plays a major role in myeloid disorders. This mutation is characterized by a G to T transverse at nucleotide 1849 in exon 12 of the JAK2 gene, located on the chromosome 9p, leading to a substitution of valine to phenylalanine at amino acid position 617 in the JAK2 protein. In this study we compared the amplification refractory mutation (ARM...
BACKGROUND A frame-shift mutation in the flagellum motor gene motB coding for the chemotaxis MotB protein of Burkholderia mallei has been utilized to design a conventional duplex PCR assay with fluorescent labelled primers. FINDINGS Species specificity was tested with a panel of 13 Burkholderia type strains. A total of 41 B. mallei field strains, 36 B. pseudomallei field strains, and 1 B. tha...
OBJECTIVES The root barks of Periploca sepium Bge. (P. sepium) has been used in traditional Chinese medicine for healing wounds and treating rheumatoid arthritis. However, toxicity in high-doses was often diagnosed by the presence of many glycosides. The potential mutagenicity of P. sepium was investigated both in vitro and in vivo. METHODS This was examined by the bacterial reverse mutation ...
Background and purpose: Nowadays, food flavorings are widely used in Iran in different food and beverage items without considering their potential threats. This research aimed at investigating directed mutagenesis caused by flavorings using the Ames Test. Materials and methods: Direct mutagenesis of five artificial food flavorings (vanilla, banana, orange, coconut, and lemon), from three diffe...
The past few years have seen a surge of novel applications of the Luria-Delbrück fluctuation assay protocol in bacterial research. Appropriate analysis of fluctuation assay data often requires computational methods that are unavailable in the popular web tool FALCOR. This paper introduces an R package named rSalvador to bring improvements to the field. The paper focuses on rSalvador's capabilit...
Background: Bacteremia due to Enterococcus faecalis is usually caused by strains resistant to most antibiotics. Effective management of the disease is dependent on rapid detection and characterization of the bacteria, and determination its sensitivity pattern to antimicrobial drugs. The aim of this study was to investigate a more rapid and reliable assay for simultaneous diagnosis of enterococc...
To the Editor: Hemochromatosis is an autosomal recessive disorder of iron metabolism affecting 0.2%– 0.5% of white populations. Approximately 90% of affected individuals are homozygous for the C282Y mutation, but the H63D and S65C mutations are also of interest. Various molecular diagnostic methods for detection of these mutations have been described, including a multiplex PCR from Stott et al....
Methods First, rhTRIM5a was examined by immunofluorescence and characterized with specific ubiquitin antibodies. Next, mutations were made in rhTRIM5a to prevent involvement in the ubiquitin-proteasome pathway, and mutants were analyzed by immunofluorescence, flow cytometry and real-time PCR. A mutant virus was constructed to prevent ubiquitination of capsid, and used to infect cells expressing...
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