The primary role of the biosensor is to specifi cally detect an analyte using biochemical reaction or interaction mediated by isolated biomolecules, organelles, whole cells or biomimetic receptors. In terms of construction and function, the biosensor consists of biorecognition element and transducer connected to suitable measurement device. Electrochemical biosensor is an electrode or microelec...

The response of an amperometric biosensor at mixed enzyme kinetics and diffusion limitations was modelled digitally in the case of substrate inhibition. Digital simulations were carried out using a finite difference technique. Calculations showed complex kinetics of biosensor response. At low enzyme activity and substrate concentration (S0), the response of the sensor looks like it is limited b...

In order to explore the clinical application of the nanobiosensor based on localized surface plasmon resonance (LSPR), we used our LSPR biosensor to detect the microalbuminuria in this work. The sliver nanoparticles were fabricated by using nanosphere lithography. The anti-human albumin antibody was immobilized on the sensor surface by amine coupling method. The different concentrations of comm...

Objective: The annual incidence of cancer in the world is growing rapidly. The most important factor in the cure of cancers is their early diagnosis. miRNA, as a biomarker for early detection of cancer, has attracted a lot of attention. Methods: In this study, an electrochemical biosensor was developed to detect the amount of miR-106a, the biomarker of gastric cancer, by modifying a glass...

F(0)F(1)-ATPase within chromatophore was constructed as a biosensor (immuno-rotary biosensor) for the purpose of capturing single virus. Capture of virus was based on antibody-antigen reaction. The detection of virus based on proton flux change driven by ATP-synthesis of F(0)F(1)-ATPase, which was indicated by F1300, was directly observed by a fluorescence microscope. The results demonstrate th...

A novel potential treatment technique applied to a glucose biosensor that is based on pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) and chromium hexacyanoferrate (CrHCF) incorporated into a platinum (Pt) electrode was demonstrated. CrHCF, serving as a mediator, was electrochemically deposited on the Pt electrode as ascertained by CV, SEM, FTIR and XPS measurements. The po...

A biosensor is defined as a measurement device that can convert a biological response into an electrical signal; this information can then be used to obtain detailed information on analyte concentration and the nature of binding to a biological or chemical receptor. Initial commercial development of biosensor technology was focused on diagnostic applications, and in 1973 Yellow Springs Instrume...

In this paper, a vertically stacked nanosheet gate-all-around field-effect transistor (GAA-NSFET) as label-free biosensor has been proposed and investigated. The influences of different biomolecules on the biosensor’s electrical properties are analyzed, exhibits good sensitivity for both neutral charged biomolecules. Furthermore, deep analysis is performed to evaluate sensing ability biosensors...