Objectives: To compare corneal endothelial cell changes namely, endothelial cell density (ECD), percentage polymegathism (CV) and pleomorphism (6A) along with central corneal thickness (CCT) in patients of type 2 diabetes mellitus with normal age and sexmatched subjects. To correlate corneal endothelial changes with diabetic retinopathy status, total duration of disease and glycemic control (Hb...

HCO-dependent fluid secretion by the corneal endothelium controls corneal hydration and maintains corneal transparency. Recently, it has been shown that mRNA for the cystic fibrosis transmembrane conductance regulator (CFTR) is expressed in the corneal endothelium; however, protein expression, functional localization, and a possible role in HCO transport have not been reported. Immunoblotting f...

hitact calf corneas and calf corneas with epithelium and endothelium removed were incubated in vitro with inorganic sulfate-S for 6 hours at 38° C. The incorporation of S into the nondialyzable substances (essentially mucopolysaccharides) was measured. No significant difference was found between the S incorporation of the denuded stroma and that of the intact cornea. Calf corneal stromas, swoll...

BACKGROUND We aimed to identify factors influencing the therapeutic outcome of orthokeratology on controlling juvenile myopia progression, and the risk factors for complications. METHODS Myopic patients (n=724) in Shenzhen Second Hospital from Jan 2011 to Jan 2016 fitted with orthokeratology lenses and followed-up for 6-65 months were reviewed retrospectively. Univariate and multivariate logi...

Corneal confocal microscopy is a novel clinical technique for the study of corneal cellular structure. It provides images which are comparable to in-vitro histochemical techniques delineating corneal epithelium, Bowman's layer, stroma, Descemet's membrane and the corneal endothelium. Because, corneal confocal microscopy is a non invasive technique for in vivo imaging of the living cornea it has...

The corneal endothelium serves primarily in maintaining stromal deturgescence which is essential for transparency of cornea. Any disturbance in its function leads to stromal edema which in turn reduces vision. The genetically heterogeneous nature of four different kinds of corneal endothelial dystrophies represents the involvement of diverse set of genes. Until now, only few genes were identifi...

AIM To generate human embryonic stem cell derived corneal endothelial cells (hESC-CECs) for transplantation in patients with corneal endothelial dystrophies. MATERIALS AND METHODS Feeder-free hESC-CECs were generated by a directed differentiation protocol. hESC-CECs were characterized by morphology, expression of corneal endothelial markers, and microarray analysis of gene expression. RESUL...

PURPOSE Hyaluronan (HA) is a disaccharide polymer capable of binding considerable amounts of water. It is present in trace amounts on the cornea endothelium, and it is not normally found in the epithelium or stroma. A specific histochemical stain was used to test for HA in a wide variety of corneal disorders. METHODS. Eighty-six human corneal tissue specimens were examined histochemically for H...

purpose: to evaluate histopathological changes induced by 0.02% mitomycin-c (mmc) in rabbits corneas. methods: this study was performed on 48 corneas of 24 rabbits (dutch type, albino). under general anesthesia, lamellar keratectomy was performed and either mmc 0.02 % (mmc group) or balanced salt solution (control group) was applied to the stromal bed for one minute. sixty days later, light mic...