Drosophila, like most insects, are susceptible to low temperatures, and will succumb to temperatures above the freezing point of their hemolymph. For these insects, cold exposure causes a loss of extracellular ion and water homeostasis, leading to chill injury and eventually death. Chill-tolerant species are characterized by lower hemolymph [Na(+)] than chill-susceptible species and this lowere...

Cryopreservation of immature testicular tissues is essential for increasing the possibilities of offspring generation by testicular xenografting for agricultural or medical purposes. However, successful production of offspring from the sperm involved has never been reported previously. In the present study, therefore, using intracytoplasmic sperm injection (ICSI), we examined whether xenogeneic...

1 This article was supported by the Florida Agricultural Experimental Station. 2 Correspondence Karen Moore, Department of Animal Sciences, University of Florida, PO Box 110910, Gainesville, Florida 326110910, USA. E-mail: [email protected] Abstract Moore K, Bonilla AQ. Cryopreservation of Mammalian Embryos: the State of the Art. ARBS Annu Rev Biomed Sci 2006;8:19-32. Embryo cryopreservatio...

Many insects in temperate regions overwinter in diapause, during which they are cold hardy. In these insects, one of the metabolic adaptations to the unfavorable environmental conditions is the synthesis of cryoprotectants/anhydroprotectants. The aim of this study was to investigate the connection between the antioxidative system and synthesis of cryoprotectants (mainly glycerol) in diapausing ...

This study reports that common carp (Cyprinus carpio) embryos were successfully frozen and preserved by vitrification. The modified Haga solution was tested by using two different cryoprotectants and three different concentrations at 4°C to obtain appropriate vitrified solutions ((dimethylsulfoxide 5, 10, 15% (DMSO) and glycerol 5, 10, 15% (gly)) in the study.  The hatching rates of embryos exp...

Background: Different cryoprotectants are used for Cryopreservation of ovarian tissue in patients who are at risk of infertility. Ethylene glycol (EG), dimethyl sulfoxide (DMSO) and propanediol (PROH) were chosen as the basic permeable cryoprotectants due to their less glass-formation characteristic compared to the others. In the present study, the effects of two different vitrification methods...

The stromal compartment of adipose tissue harbors multipotent cells known as adipose-derived stem cells (ASCs). These cells can differentiate into various lineages including osteogenic, chrondrogenic, adipogenic, and neurogenic; this cellular fraction may be easily obtained in large quantities through a clinically safe liposuction procedure. Therefore, ASCs offer exceptional opportunities for t...

Recently, increasing attention has been paid to lecithin/chitosan nanoparticles as colloidal drug delivery system showing the great potential to improve the permeation of encapsulated drug across various biological barriers [1, 2]. However, the overall poor stability of colloidal nanoparticles in an aqueous medium is a significant drawback in their development perspective. Therefore, the aim of...

Loading of cryoprotectants into oocytes is an important step of the cryopreservation process, in which the cells are exposed to potentially damaging osmotic stresses and chemical toxicity. Thus, we investigated the use of physics-based mathematical optimization to guide design of cryoprotectant loading methods for mouse and human oocytes. We first examined loading of 1.5 M dimethyl sulfoxide (M...