Debate still surrounds the physiological roles of the alternative respiratory enzymes found in many fungi and plants. It has been proposed that alternative NADH:ubiquinone oxidoreductases (NADH dehydrogenases) may protect against oxidative stress, conversely, elevated activity of these enzymes has been linked to senescence. Here we show that inhibition of these enzymes in a fungal protein expre...

A method for the purification of glutamate dehydrogenase from dogfish liver has been presented. The molecular weight of the enzyme has been determined and the sedimentation constant has been found to be invariant over a wide range of concentrations, in contrast to the bovine liver glutamate dehydrogenase. Upon electrophoresis the dogfish enzyme has been observed to migrate further than the beef...

12-Keto-UDCA is an important optically active component for the drug ursodeoxycholic acid (UDCA). Starting from the three-keto compound dehydrocholic acid, the carbonyl groups at position 3 and 7 have to be reduced stereoand regioselectively. In this case we applied two hydroxysteroid dehydrogenases for this purpose, the NAD-dependent 3α-HSDH from Pseudomonas testosteroni and the NADP-dependent...