Gene therapy targeted at the respiratory epithelium holds therapeutic potential for diseases such as cystic fibrosis and lung cancer. We recently reported that Chitosan-DNA-FAP-B nanoparticles are good candidates for targeted gene delivery to fibronectin molecules (FAP-B receptors) of lung epithelial cell membrane. In this study Chitosan-DNA-FAP-B nanoparticles were nebulized to mice using air ...

Nucleic acids are biologically active materials, and chemically modified nucleic acids are now being used in nucleic acid drugs. DNA, one of the two types of nucleic acids, can also be used as a material to construct DNA-based delivery systems, such as DNA hydrogel, for therapeutic compounds. Use of chemically modified nucleic acids would greatly increase the therapeutic potency of such DNA-bas...

BACKGROUND Preterm delivery and sub-optimal fetal growth are associated with each other and affect both mother and infant. Our aim was to determine (i) whether there are detectable differences in DNA methylation between early and late gestation and (ii) whether changes in DNA methylation from entry are associated with spontaneous preterm delivery with and without reduced fetal growth. METHODS...

Cell free fetal DNA (cff DNA) is apoptotic that shed from placenta and flows in maternal blood. This can be used as an indicative biomarker to rule out pregnancy associated complications. The objective of this study investigate the correlation concentration cff with intrauterine deaths. RHD gene (Rh blood group, D antigen) was marker for confirmation GAPDH (Glyceraldehyde-3-phosphate dehydrogen...

background hypervariability of hcv proteins is an important obstacle to design an efficient vaccine for hcv infection. multi-epitope vaccines containing conserved epitopes of the virus could be a promising approach for protection against hcv. objectives cellular and humoral immune responses against multi-epitope dna and peptide vaccines were evaluated in balb/c mice. materials and methods in th...

How to cite this article Hu J, Bounds CE, Budgeon LR, Cladel NM, Balogh K and Christensen ND. DNA Vaccines Delivered By Microneedle and Tattoo Gun Induce Protective Immune Responses to Hla-A2.1 Restricted CRPV E1 and HPV16e7 Epitopes in HLA-A2.1 Transgenic Rabbits. SM Vaccine Vaccin. 2016; 2(1): 1017. OPEN ACCESS Gene gun delivery of DNA vaccines has proven to be effective for protective and th...

The safe, targeted and effective delivery of gene therapeutics remains a significant barrier to their broad clinical application. Here we develop a magnetic nucleic acid delivery system composed of iron oxide nanoparticles and cationic lipid-like materials termed lipidoids. Coated nanoparticles are capable of delivering DNA and siRNA to cells in culture. The mean hydrodynamic size of these nano...