Experiments concerning tumor transformation of plant cells or protoplasts need complete knowledge of the effects of media components. Here inhibiting effects of cellulase Onozuka, glucose, sucrose and mannitol (up to 0.3M), of tissue culture media, and of the antibiotic aureomycin on the tumor initiation process in Kalanchoe leaves are reported. Also the influence of these substances on the gro...

Plants growth regulators, especially auxins and cytokinins are critical for in-vitro plant regeneration. The effect of plant growth regulators on the in-vitro propagation of Senna alata (Linn) was investigated. Mature seeds were collected from the medicinal garden of National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan (Lat.7022I N and long.3050I E). Seeds of the species we...

A novel approach to studying the production of secondary metabolites by fungi using LC/TOF-MS has been developed. Fungi grown on culture media are solvent-extracted and directly analyzed by LC/TOF-MS. Searching against a database of 465 secondary metabolites, mycotoxins and other compounds of interest can be readily identified. The methodology was validated by spiking culture media with 20 myco...

In vitro techniques may provide a suitable tool for effective propagation and conservation of plant species representing various ecological niches. The elaboration of such protocols is also prerequisite for selection of heavy-metal-tolerant plant material that could be afterwards used for restoration or remediation of polluted sites. In this study, culture protocol for Gypsophila fastigiata pro...

In order to identify the endophytic fungi of stone fruit trees in West Azarbaijan province (Iran), healthy tissues were collected from various regions. For isolation of endophytic fungi, plant tissues were primarily washed with tap water, then surface sterilized by rinsing in 75% ethanol for 1 minute, followed by 5 minutes in 3% sodium hypochlorite (active ingredient). Disinfested pieces were t...

the development of culture media able to mimic the preovulatory stage of follicular environment and support nuclear and cytoplasmic maturation of oocyte is important for in-vitro fertilization (ivf) programs. it seems that the best culture media for embryonic development and cell cleavage is the natural composition which surrounds the oocyte which has been used occasionally in human ivf program...

Microbial cells are producers of natural polymers present in plant cells. Production of pullulan (an extracellular microbial polysaccharide) by Aureobasidium pullularia pullulans (P. pullulans) was studied under fermentation conditions, and kinetic parameters were determined. Pullulan formation obeyed a growth and non-growth associated term. PHB (polyhydroxybutyrates) an intracellular biopo...