background and aims: the causal agents of viral lettuce big vein disease are two viruse, lettuce big vein associated virus (varicosavirus) and mirafiori lettuce virus (ophiovirus). these viruses have coat proteins of similar size but have different morphologies and serologically unrelated.the purpose of this study was to distinguish and detect lbvav and milv in lettuce fields in tehran province...

The present study was carried out to detect the presence of Alfalfa mosaic alfamovirus (AMV), cucumber mosaic cucumovirus (CMV), lettuce mosaic potyvirus (LMV), cucumber green mottle mosaic virus (CGMMV), tomato bushy stunt tombusvirus (TBSV), tobacco mosaic tobamovirus (TMV), tomato black ring nepovirus (TBRV) and tomato mosaic tobamovirus (ToMV) in seeds of pepper, tomato, cucumber and lettuc...

Based on the design of the ELISA calibration in the comparability study CCQM-P58, three measured signals are simulated at each of the 11 known concentrations (100, 50, . . . , 0.195, 0) ng/ml. These calibration data are simulated according to Model (1.1) using the parameters β = (1.466, 0.1024, 67.93, 1.361), a = 3.933 × 10, c = 9.710 × 10, and are listed in Table 1. Based on the comparability ...

Enzyme-linked immunosorbent assay (ELISA) and micro-ELISA were evaluated for their ability to detect anti-Fasciola hepatica antibodies in humans by using excretory-secretory antigen. The sensitivity of each method was 100%, but the specificity was 100% for ELISA and 97% for micro-ELISA. The micro-ELISA could be used as a screening assay and ELISA could be used as a confirmatory method for the s...

ABSTRACT: The fluorescence polarization assay (FPA), two variants (V) of the indirect enzyme-linked immunosorbent (I-ELISA) and competitive (C-ELISA) were evaluated in buffaloes to detect antibodies against Brucella spp. V1 I-ELISA identifies them through monoclonal (M23) anti-bovine IgG (I-ELISAM23) V2 ProteinA / G (I-ELISA-A/G). Serum samples 862 (Bubalus bubalis) from Northeast Argentina (NE...