a rapid and simple technique for diagnosis of rotaviral diarrhea is polyacrylamide gel electrophoresis (page) of dsrna extracted from fecal samples. to determine the electrophoretype of iranian calf rotavirus, 23 rotaviral positive fecal samples from diarrheic holstein calves at the age of less than one month in tehran region were examined. the viral dsrna was extracted with phenol-chloroform, ...

idiarrhea is known as the current and economic disease among new born calves. it is characterized by dehydration, loss of macro-minerals, weakness, recumbency and death. in calves diarrhea with different etiology, loss of liquid and electrolytes are the principle signs and the cause of death too. therefore, determination of the amounts of liquid and mainly electrolytes depletion is important in...

There is a great need to detect gastrointestinal tract cancer at an early stage. It is well known that most carcinoma tissues of the gastrointestinal tract contain carcinoembryonic antigen (CEA). Stools are a rich source of cells derived from the gastrointestinal tract. We analyzed total fecal CEA in 60 gastrointestinal tract cancer patients, 20 benign gastrointestinal tract disorder patients, ...

shiga toxin-producing escherichia coli (stec) are food-borne pathogens primarily associated with the consumption of contaminated ground beef and are an important food safety concern worldwide. stec has been found to produce a family of related cytotoxins known as shiga toxins (stxs). shiga toxins have been classified into two major classes, stx1 and stx2. a single strains of stec can produce st...

Jugular catheters were inserted in nine male rats under general isofluorane anesthesia and the catheters were connected to a commercially available computerized blood sampling device (Accusampler). Blood samples (150 microl) were collected every 4 h during the first 24 h after surgery and every 12 h during the following 72 h until 94 h after surgery, when the animals were killed. All fecal pell...

BACKGROUND Samples of drinking water were collected directly from the personal water bottles of students at an elementary school in Calgary, Alberta. METHODS Total and fecal coliforms and heterotrophic bacteria were enumerated using membrane filtration and agar plate count methods respectively. RESULTS The Canadian Drinking Water Quality Guidelines (CWQG) criterion was exceeded for total co...

The aim of the present study was to determine seroprevalence of Toxoplasma gondii infectionin stray cats and correlation with oocyst shedding and IFN-γ concentration. From April to August 2016, one hundred fifty-nine stray cats were captured from various localities in Mashhad area. The blood and fecal samples were collected from each cat. The serum samples were examined to detect antib...

Fasciolosis is a zoonotic parasitic disease with worldwide distribution. The disease causes significant economic losses in herd industry including decrease animal products such as meat, milk and infertility reduction. Human can acquire infection as accidental host especially in areas with high level of raining and humidity. Previous studies showed the prevalence of infection among meat-product ...

Assays for the detection and typing of adenoviruses, enteroviruses and F+ specific coliphages were performed on samples created as part of a national microbial source tracking methods comparison study. The samples were created blind to the researchers, and were inoculated with a variety of types of fecal contamination source (human, sewage, dog, seagull and cow) and mixtures of sources. Viral t...