The 62 kDa FG repeat domain of the nucleoporin Nsp1p forms a hydrogel-based, sieve-like permeability barrier that excludes inert macromolecules but allows rapid entry of nuclear transport receptors (NTRs). We found that the N-terminal part of this domain, which is characterized by Asn-rich inter-FG spacers, forms a tough hydrogel. The C-terminal part comprises charged inter-FG spacers, shows lo...

This study was undertaken to identify genetic polymorphisms that are associated with the risk of an elevated fasting glucose (FG) level using genome-wide analyses. We explored a quantitative trait locus (QTL) for FG level in a genome-wide study from a Korean twin-family cohort (the Healthy Twin Study) using a combined linkage and family-based association analysis approach. We investigated 1,754...

In the desert locust, Schistocerca gregaria, the frontal ganglion (FG) plays a key role in control of foregut movements, and constitutes a source of innervation to the foregut dilator muscles. In this work we studied the generation and characteristics of FG motor outputs in two distinct and fundamental behaviours: feeding and moulting. The FG motor pattern was found to be complex, and strongly ...

The present study reports on fibrinogen (Fg) binding of Staphylococcus epidermidis. Adhesion of different S. epidermidis strains to immobilized Fg was found to vary significantly between different strains, and the component responsible was found to be proteinaceous in nature. To further characterize the Fg-binding activity, a shotgun phage display library covering the S. epidermidis chromosome ...

This study examined the effects of storage conditions such as time course, temperature, fluorescent light, and darkness on the components and antibacterial activity of formalin guaiacol (FG) used in endodontic treatment. We measured the quantities of formaldehyde and guaiacol in FG and antibacterial activities against Staphylococcus aureus, Porphyromonas gingivalis, and Porphyromonas endodontal...

A silicon nanosensor technology based on electrical impedance measurements has been developed for the detection of proteins. The nanosensor miniaturizes the high-density, low-volume multiwell plate concept. The scientific core of this technology lies in integrating nanoporous membranes with microfabricated chip platforms. This results in the conversion of individual pores into nanowells of pico...

This paper details the development of the fixed grid FEA (FGFEA) method to the solution of eigenvalue (natural frequency and buckling) problems, using a 4 node 20 degree of freedom shell element. The formulation of the FG element stiffness, buckling and natural frequency equations is presented. The first natural frequency and buckling load are determined for the simply supported plate with a ce...

In this study, the mechanical buckling response of refined hyperbolic shear deformable (FG) functionally graded nanobeams embedded in an elastic foundation is investigated based on the refined hyperbolic shear deformation theory. Material properties of the FG nanobeam change continuously in the thickness direction based on the power-law model. To capture small size effects, Eringen’s nonlocal e...

Abstract Nuclear pore complexes (NPCs) regulate all molecular transport between the nucleus and cytoplasm in eukaryotic cells. Intrinsically disordered Phe-Gly nucleoporins (FG-Nups) line central conduit of NPCs to impart a selective barrier where large proteins are excluded unless bound receptor (karyopherin; Kap). Here, we assess “Kap-centric” NPC models, which postulate that Kaps participate...

We applied transmission electron microscopy and densitometric image analysis to measure the cell volume (V) and dry weight (DW) of single bacterial cells. The system was applied to measure the DW of Escherichia coli DSM 613 at different growth phases and of natural bacterial assemblages of two lakes, Piburger See and Gossenköllesee. We found a functional allometric relationship between DW (in f...