It is well known that eating fruits and vegetables lowers the risk of chronic diseases such as heart disease and cancer. The question of what is/are the active ingredient(s) is still unresolved. The initial hypothesis was that the antioxidant vitamins were responsible. However, recently the polyphenols have been investigated since they have been found to have beneficial properties such as being...

In the present work, an automatic flow procedure for the sequential spectrophotometric determination of Folin-Ciocalteu reducing capacity (FC assay) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS*+) scavenging capacity expressed as the trolox equivalent (TEAC assay) is proposed for a comparative study of antioxidant properties in food products. Exploiting the fle...

BACKGROUND AND OBJECTIVES Proteases are a group of enzymes that catalyze the degradation of proteins resulting in the production of their amino acid constituents. They are the most important group of industrial enzymes which account for about 60% of total enzymes in the market and produced mainly by microorganisms. The attempts were made to study the kinetic parameters of protease produced by S...

The reaction of picric acid with creatinine in alkaline solution (Jaffe’s reaction), which is the basis of the Folin method (1) for the determination of creatinine, has been thought to be either a reduction of the acid or else the formation of a red tautomer of creatinine picrate. Greenwald (2) obtained evidence that the tautomerism involves picric acid as well as creatinine, and that other nit...

INTRODUCTION Hyssopus officinalis (L) (Hyssop, Family: Lamiaceae), one of the endemic Iranian perennial herb with a long history of medicinal use, was studied to detect some biologically active chemical constituents of the plant. METHODS The flavonoids of the hydromethanolic extract of the aerial parts of Hyssopus officinalis (L.) were studied by VLC and crystalisation of the major compound i...

Eight brands of tea (Camellia sinensis),which are cultivated and commercially available in Ethiopian market, were analyzed for estimation of their total secondary metabolites (polyphenols, flavonoids and tannins) content and free radical scavenging activity which is expressed on dry weight basis. In this present study, the total polyphenols, tannin and flavonoid contents were studied spectropho...

FOR some years past much attention has been directed to the determination of carbohydrate tolerance by observing the variations that occur in the sugarcontent of the blood after the ingestion of a known amount of sugar. The value of this method is now universally recognised. It is not intended in this paper to treat the subject in a general way, but merely to consider the actual estimation of s...

In recent years the concept of the specificity of enzyme action has been subjected to revision. The proteolytic enzymes (1) have been shown to have esterase activity and the concept of a blood esterase as distinct from a blood lipase is no longer tenable (2). The enzymatic hydrolysis of the steroid hormone esters by blood sera (3) had not been suspected until solubility determinations of the es...