3'(2')-O-(4-Benzoyl)benzoyl-ATP (Bz2ATP) was used as a photoaffinity label of the ATP binding site of unphosphorylated chicken gizzard myosin. Specific photolabeling of the active site of 6 S myosin was assured by forming a stable myosin.Co(II)Bz2ADP.orthovanadate complex (termed trapping) prior to irradiation. Co2+ was used in place of Mg2+ to prevent the known photoreaction of vanadate with m...

The relationship between nutritional status and insulin-like growth factor binding protein-2 (IGFBP-2) gene expression in chickens was studied. Chickens (6 wk old) were food deprived for 2 d and then refed. IGFBP-2 mRNA in the brain was significantly decreased by food deprivation and levels did not increase when birds were refed for 24 h. Gizzard and hepatic IGFBP-2 mRNA levels were significant...

The current National Research Council (NRC) selenium (Se) requirement for the turkey is 0.2 μg Se/g diet. The sequencing of the turkey selenoproteome offers additional molecular biomarkers for assessment of Se status. To determine dietary Se requirements using selenoprotein transcript levels and enzyme activities, day-old male turkey poults were fed a Se-deficient diet supplemented with graded ...

Artificial selection in closed populations can fix or differentiate alternative alleles of loci associated with selected traits. Two closed chicken populations, A and B, originating from Jiuwu, a Chinese native breed, were bred for more than 10 generations. We compared progeny from reciprocal crosses (AB and BA) under free range (trial 1) and cage and pen (trial 2) systems. Traits measured incl...

Over the past five decades, a reproductive failure related to thiamine deficiency, referred to as thiamine deficiency complex (TDC), has been observed in valuable salmonine fishes in the Great Lakes and Finger Lakes in North America and the Baltic Sea in Europe. The cause of TDC has been linked to the consumption of clupeid fish, which contain high levels of a thiamine-destroying enzyme called ...

December 2006 | Volume 4 | Issue 12 | e418 Most governments around the world set conservation policy based on the assumption that resource exploitation and species protection can co-exist in the same place. These policies have led to Orwellian “marine protected areas” that host commercial fi shing operations, leading one to wonder who’s protecting whom. A new study reveals the danger of this ap...

December 2006 | Volume 4 | Issue 12 | e418 Most governments around the world set conservation policy based on the assumption that resource exploitation and species protection can co-exist in the same place. These policies have led to Orwellian “marine protected areas” that host commercial fi shing operations, leading one to wonder who’s protecting whom. A new study reveals the danger of this ap...

December 2006 | Volume 4 | Issue 12 | e418 Most governments around the world set conservation policy based on the assumption that resource exploitation and species protection can co-exist in the same place. These policies have led to Orwellian “marine protected areas” that host commercial fi shing operations, leading one to wonder who’s protecting whom. A new study reveals the danger of this ap...