نتایج جستجو برای: indirect immuno fluorescence assay
تعداد نتایج: 400642 فیلتر نتایج به سال:
For the evaluation of autoantibodies in Pemphigus vulgaris (PV), an indirect ELISA assay was developed by using a semi-purified human epidermal skin extracted with approximately a molecular weight (M.W.) of 130-160 kDa. The evaluation of Pemphigus IgG, IgM and IgA autoantibodies in 75 patients and 50 control sera indicated that this indirect ELISA assay was a useful method for the detection of ...
In eukaryotes, vesicle trafficking is regulated by the small monomeric GTPases of the Rab protein family. Rab11, (a subfamily of the Ypt/Rab gene family) an evolutionarily conserved, ubiquitously expressed subfamily of small monomeric Rab GTPases, has been implicated in regulating vesicular trafficking through the recycling of endosomal compartment. In an earlier communication, we have shown th...
The calcium/proton antiporter of Escherichia coli was characterized by measurement of the effect of divalent cations on the respiration-coupled quenching of the fluorescence of quinacrine or 9-aminoacridine in everted membrane vesicles. Energy-linked quenching of the fluorescence of aminoacridines is dependent on proton uptake into everted vesicles and provides an indirect assay for measurement...
Classical serological screening assays for Chagas' disease are time consuming and subjective. The objective of the present work is to evaluate the enzyme immuno-assay (ELISA) methodology and to propose an algorithm for blood banks to be applied to Chagas' disease. Seven thousand, nine hundred and ninety nine blood donor samples were screened by both reverse passive hemagglutination (RPHA) and i...
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