1Unit of Molecular Biology and Viral Oncology, Department of Experimental Oncology, Istituto Nazionale Tumori IRCCS Fondazione Pascale, 80131 Napoli, Italy 2Department of Microbiology and Immunology, Institute of Human Virology, Baltimore, MD 21201, USA 3Department of Research, International Prevention Research Institute, 69006 Lyon, France 4Department of Pathology, Istituto Nazionale Tumori IR...

as recent advancements in biology shows, the molecular machines specially proteins, rna and complex molecules play the main role of the so called cell functionality. it means a very big part of the system biology is concerned with the interactions of such molecular components. drug industries and research institutes are trying hard to better understand the concepts underlying these interactions...

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White-rot basidiomycetes are one of the most important lignolytic microorganisms. These fungi have been reported to secrete three main classes of lignin degrading enzymes: lignin peroxidases (LiPs), manganese peroxidases (MnPs) and laccases. In this study, for the first time the lignin degrading capability of two plant pathogens i.e. Hyphodermella rosae and H. corrugata was evaluated using both...

Background & Aims: Cryptosporidiosis is one of the most important parasitic zoonoses of human and animals. This infection is common in mammals and caused by the coccidian parasites of the genus Cryptosporidium. The Present study was designed to determine the epidemiology of Cryptosporidium infection in cattle in Kerman by using conventional morphological as well as molecular methods for molecul...

Medical Mycology is first and foremost a laboratory technique manual. While each chapter begins with some explanatory text providing background on the pathogenic fungi, the bulk of the book is devoted to protocol. The text, edited by Kevin Kavanagh, covers diagnosis ofCandida infection by immunohistochemistry, techniques for identification of pathogenic fungi using transmission electron microsc...

In many bird species, males and females cannot be discriminated on the basis of external characteristics. The problems associated with reliable sex identification were overcome by introducing the molecular techniques in the 1990s. These techniques are generally based on DNA hybridization or polymerase chain reaction (PCR). This paper reviews the sexing techniques, highlighting their advantages ...

Site-directed mutagenesis: colony growth This month's question from the Molecular Biology Forums (online at molecularbiology. forums.biotechniques.com) comes from the " General Methods " section. Entries have been edited for concision and clarity. Mentions of specific products and manufacturers have been retained from the original posts, but do not represent endorsements by, or the opinions of,...

The nucleic acid hybridization is the process wherein two DNA or RNA single chains (mono-stranded) from different biological sources, make the double catenary configuration, based on contingent sequence homology of the two sources, resulting DNA-DNA, RNA-RNA or DNA-RNA hybrids. The purpose is identification or localization of certain nucleic acid sequences (genes) in the genome of some species....