According to WHO one third of the world population have tuberculosis. The present study was undertaken to compare the efficacy of flurochrome(FI) stain with conventional Z-N stain in the diagnosis of pulmonary & extrapulmonary tuberculosis. 388 cases of suspected pulmonary tuberculosis were included in the study. All samples were screen for Acid Fast Bacilli (AFB) by Z-N & Fluorescent staining ...

Tuberculosis (TB) is an important public health issue in this world. In 1992, World Health Organization (WHO) has determined tuberculosis as Global Emergency. The caused of tuberculosis disease is Mycobacterium tuberculosis. This bacteria is bar shaped and has the quality of acid fast so that it’s also called as Acid Fast Bacilli (AFB). In this research, an algorithm to identifying and counting...

Background: Microbiological confirmation of tuberculous meningitis (TBM) remains problematic. We assessed the diagnostic performance of a modified Ziehl-Neelsen (MZN) staining method that showed promise in earlier studies. Methods: Patients evaluated for TBM in Shaanxi province, China, were prospectively enrolled from May, 2011 to April, 2013. Cerebrospinal fluid (CSF) specimens were evaluated ...

Objectives: Hansen’s disease is a chronic granulomatous infectious caused by Mycobacterium leprae. Early detection essential to prevent the disease’s spread and disability. Due multitude of morphologies, it may be difficult accurately diagnose certain cases based solely on clinical signs; therefore, histopathology crucial for definitive diagnosis. The study aims investigate various histological...

In this study, a case of pulmonary aspergillosis was described in 20-day-old suckling lamb. Lamb suffering from high fever, anorexia, weakness and balance disorder brought to the Department Pathology Kafkas University Faculty Veterinary Medicine for necropsy. According anamnesis obtained animal owner, an intense random drug treatment administered animal. Following systemic necropsy animal, vari...

The Ziehl–Neelsen (ZN) stain, also known as the acidfast stain, has been reported to be helpful in detection and identification of Schistosoma eggs. In histological sections, Schistosoma mansoni egg shells appear as ZN positive and Schistosoma haematobium shells as ZN negative. The staining target of the responsible ZN component (carbolfuchsin) in the shell is unknown. Because carbolfuchsin is ...

OBJECTIVE To determine the accuracy of Ziehl-Neelsen microscopy in the diagnosis of TB in setings of high HIV prevalence. DESIGN Cross-sectional descriptive study. SETTING Hospitals serving areas of high human immunodeficiency virus prevalence in western Kenya. The study was conducted between September 2007 and September 2009. RESULTS In total, 341/872(39.1%) of the TB suspects were posit...

Screening for tuberculosis in high-prevalence countries relies on sputum smear microscopy. We present a method for the automated identification of Mycobacterium tuberculosis in images of Ziehl-Neelsen-stained sputum smears obtained using a bright-field microscope. We use two stages of classification. The first comprises a one-class pixel classifier for object segmentation. Geometric transformat...