Small interfering RNAs (siRNAs) are promising new active compounds in gene medicine but the induction of non-specific immune responses following their delivery continues to be a serious problem. With the purpose of avoiding such effects chemically modified siRNAs are tested in screening assay but often only examining the expression of specific immunologically relevant genes in selected cell pop...

The biological activity of 6mercaptopurine, an analogue of hypoxanthme, has been the subject of extensive studies, but the mechanism of its action has remained unclear. This drug inhibits the growth of bacteria (l-3) and mammalian cells (4, 5) in vitro and the proliferation of experimental tumors and leukemias in tiuo (6-9). It has been suggested that it interferes with the synthesis of nucleic...

Aptamers are short nucleic acid oligos. They may serve as both drugs and drug-carriers. Their use as diagnostic tools is also evident. They can be generated using various experimental, theoretical, and computational techniques. The systematic evolution of ligands by exponential enrichment which uses iterative screening of nucleic acid libraries is a popular experimental technique. Theory inspir...

background: we examined a molecular method with a single-pcr for amplification of a part of cp5 gene enabling us to differen­tiate the pathogenic species, entamoeba histolytica , from the non-pathogenic species, e. dispar . methods: we developed a single pcr method for this purpose.  after investigation of genbank, primer pairs were de­signed from highly conserved regions of cysteine proteinase...

The sesquiterpenoids nerolidol, farnesol, bisabolol, and apritone were investigated for their abilities to enhance bacterial permeability and susceptibility to exogenous antimicrobial compounds. Initially, it was observed by flow cytometry that these sesquiterpenoids promoted the intracellular accumulation of the membrane-impermeant nucleic acid stain ethidium bromide by live cells of Lactobaci...

Hypochlorous acid (HOCl) produced by myeloperoxidase (MPO) of activated neutrophils can react with nucleic acid bases to form chlorinated nucleosides such as 8-chloroguanosine (Cl-Guo). Chlorination is enhanced by nicotine. We investigated the effects of various natural antioxidants including polyphenolic phytochemicals on the formation of Cl-Guo by HOCl in the presence and the absence of nicot...

Svihla, G. (Argonne National Laboratory, Argonne, Ill.), J. L. Dainko, and F. Schlenk. Ultraviolet microscopy of purine compounds in the yeast vacuole. J. Bacteriol. 85:399-409. 1962.-Yeast cells (Candida utilis and Saccharomyces cerevisiae) suspended in nitrogen-free medium were exposed to various ultraviolet-absorbing biological compounds, particularly nucleic acid constituents. Ultraviolet p...

Nucleic acids and carbohydrates are essential biomolecules involved in numerous biological and pathological processes. Development of multifunctional building blocks based on nucleosides and sugars is in high demand for the generation of novel oligonucleotide mimics and glycoconjugates for biomedical applications. Recently, aminooxyl-functionalized compounds have attracted increasing research i...

We identified Alizarin Red S and other well known fluorescent dyes useful for the online detection of pyrophosphate in enzymatic assays, including the loop mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assays. An iterative screening was used for a selected set of compounds to first secure enzyme compatibility, evaluate inorganic pyrophosphate sensitivity in the pr...

A number of N-substituted thymine and adenine derivatives, 2a, b and 3a, b, were synthesized by the coupling reaction of 1-bromo-2,2-diethoxyethane with the corresponding base. The corresponding peptide nucleic acid (PNA) analogues, N-substituted ethylamino-3-hydroxypropanoate derivatives 5a, b and ethylamino-3-hydroxybutanoate derivatives 6a, b, were synthesized from the corresponding 2-[3,4-d...