Novel sources of urease from leguminosae were selected for activity test, one the them (Prosopis farcta) showed high specific activity (36.52 U mg). The active uerase extract then subjected to series of purification steps involved salting out, DEAE, CM ion exchange and size exclusion chromatography. Specific activity were 52.44, 193.27, 230.55, 433.33 U mg respectively. Main features of urease ...

Cytochrome oxidase probably occurs in the tissues of most plants and mediates a major portion of terminal respiration. Webster (11, 12) recently demonstrated the presence of a cytochrome oxidase in the tissues of 54 plant species, representing 23 families of dicotyledonous plants. Investigations in the plant physiology laboratory at Rutgers University have indicated the presence of a cytochrome...

Type of dietary direct-fed microbials (DFMs) or poultry litter could directly influence the composition of gut microbiota. Gut microbiota plays an important role in shaping the developing immune system and maintaining the homeostasis of the mature immune system in mammal and chickens. The present study was carried out to investigate the interaction among litter, DFMs and immunity in broiler chi...

Clinical chicken coccidiosis is mostly caused by simultaneous infection of several Eimeria species, and host immunity against Eimeria is species-specific. It is urgent to identify common immunodominant antigen of Eimeria for developing multivalent anticoccidial vaccines. In this study, sporozoite proteins of Eimeria tenella, Eimeria acervulina and Eimeria maxima were analyzed by two-dimensional...

Normal calf ao-mannosidase activity exists in at least three forms separable by chromatography on DEAE-cellulose and by starch-gel electrophoresis. Two components, A and B, have optimum activity between pH 3.75 and 4.75, but component C has an optimum of pH 6.6. Components A and B are virtually absent from the tissues of a calf with mannosidosis and the residual activity is due to component C. ...

BACKGROUND Intensive poultry production systems depend on chemoprophylaxis with anticoccidial drugs to combat infection. A floor-pen study was conducted to evaluate the anticoccidial effect of Artemisia annua and Foeniculum vulgare on Eimeria tenella infection. Five experimental groups were established: negative control (untreated, unchallenged); positive control (untreated, challenged); a grou...

ABSTRACT Natural sources are microbiological species and medicinal plants, which could be potential new for development of drugs against different diseases. Coccidiosis affects many animals leads to great economic losses. Drug-resistant strains Eimeria have emerged because overuse misuse drugs. In vitro, using papillata oocyst earthworm (Eisenia fetida), we evaluated the anticoccidial anthelmin...

The activity of phospholipase C in crude enzymatic preparation of Melia azadiracht seeds (Neem seeds) was studied by the use of lecithin as a substrate in aqueous medium. The enzyme activity was found optimum at pH 2.5 and temperature 35?C. The phospholipase C was found heat labile, being inactivated 88% within 10 minutes at 90°C