We purified from bovine serum a glycoprotein that promotes the survival of rat embryonic neurons cultured from septum and other brain regions. A 40,000-fold purification was achieved by using a combination of ammonium sulfate precipitation, Zn2+ affinity chromatography, Cibacron blue 3-GA dye affinity chromatography, ABx ion exchange chromatography, and preparative PAGE. The active protein had ...

The isolated fungi Myrothecium spp MRP001 represent an important source of biologically active compounds. The maximum biomass production was optimized (pH 6.5, 96 h at 30 °C) using OFAT (one factor at time) in six different medias, After 96 h of growth, Tryptone Soya Broth (TSB) and Potato Dextrose Broth (PDB) shows maximum spore account (2×10 8 CFU/ml) of Myrothecium spp MRP001. Screening of b...

A NAD(P)H oxidizing system (NAAP) was detected and partially purified from leaves of spinach and Sedum praealtum, seeds and leaves of pea and cells of green and red algae which oxidized NAD(P)H in the presence of ascorbate and H(2)O(2).The partially-purified spinach system had substrate K(m) values of 5 micromolar for NADH, 50 micromolar for H(2)O(2), and 300 micromolar for l-ascorbic acid at t...