نتایج جستجو برای: pcr reaction is almost twice compared with rt
تعداد نتایج: 11403121 فیلتر نتایج به سال:
Avian hepatitis E virus (HEV) isolates could be separated into at least three genotypes. In this study, the development of the first duplex TaqMan real-time reverse transcription-PCR (RT-PCR) assay for detection and quantification of avian HEV is presented. Primers and probes binding within relatively conserved open reading frame 3 (ORF3) were designed. Tenfold dilution series of in vitro-trans...
background: establishment of viable pregnancy requires embryo implantation and placentation. ectopic pregnancy (ep) is a pregnancy complication which occurs when an embryo implants outside of the uterine cavity, most often in a fallopian tube. on the other hand, an important aspect of successful implantation is angiogenesis. vascular endothelial growth factor (vegf) is a potent angiogenic facto...
The real-time polymerase chain reaction (RT-PCR), also called quantitative real-time polymerase chain reaction (QRT-PCR) or kinetic polymerase chain reaction (kPCR), is a technique used to simultaneously quantify and amplify a DNA molecule. It is used to determine whether a specific DNA sequence is present in the sample; and if it is present, the number of copies in the sample. It is the real-t...
deposite the different criticisms on contrastive analysis it has been proved that the results of it(when processed)can be usuful in a tefl environment,specially at the level of phonology.this study is an attempt to compare and contrast the sound systems of kurdish and english for pedagogical aims. the consonants,vowels,stress and intonation of the twolanguages are described by the same model-ta...
Objective: To determine the diagnostic value of rapid COVID-19 antigen test (Panbio, Abbott) in comparison with Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) suspected community cases SARS-CoV-2 infection at a tertiary care center Pakistan 
 Study Design: Cross sectional study.
 Place and Duration Study: Pathology Department, Combined Military Hospital, Lahore Pakistan, fro...
fluorescent monitoring of dna amplification is the basis of real-time pcr. absolute quantification can be achieved using a standard curve method. the standard curve is constructed by amplifying known amounts of standards under identical conditions to that of the samples.the objective of the current study is to propose a mathematical model to assess the acceptability of pcr resulys.four commerci...
this study purported to compare and contrast the use of self-mention and evidentials as two mtadiscourse features in opinion columns of persian and english newspapers. the theoretical basis of this study is the idea that metadiscourse features vary across cultural boundaries. for this purpose, 150 persian and 150 english opinion columns were collected based on three factors of topic, audience a...
With the introduction of real-time, fluorescence-based 5′ nuclease PCR (3,4) and instruments such as the ABI PRISM 7700 (TaqMan) sequence detector (Applied Biosystems, Foster City, CA, USA), quantitative RT-PCR is now a widely accepted method for measuring gene expression levels. Quantitative RTPCR is a sensitive technique and is particularly useful for the analysis of samples containing limi...
A novel multitarget real-time PCR (RT-PCR) assay for the rapid identification of Bordetella pertussis, B. parapertussis, and B. holmesii was developed using multicopy insertion sequences (ISs) in combination with the pertussis toxin subunit S1 (ptxS1) singleplex assay. The RT-PCR targets for the multiplex assay include IS481, commonly found in B. pertussis and B. holmesii; IS1001 of B. parapert...
in this research the focus of the researcher is on the influence of american myths that have long been held and offered by the american culture and ideologies. sam shepard is conceived to be one of the greatest playwrights of the contemporary american theater. in his works he depicts broken characters who are struggling to keep up with the national expectations but are ceaselessly borne back to...
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