hepatitis b virus (hbv) is considered as a global health concern and hepatitis b surface antigen (hbsag) is the most immunogenic protein of hbv. the purpose of this study was to evaluate the expression of hbsag on the cell surface of human embryonic kidney cell line (hek293t). after transformation of expression vector pcdna/hbsag to e.coli top10f’, plasmid was extracted and digested with bglii....

the gene encoding the human basic fibroblast growth factor (hbfgf) has been already chemically-synthesized and cloned in pet-3a expression vector (pasteur institute of iran). in the present study, we compared the level of expression of this synthetic hbfgf and its related cdna in escherichia coli. the pbr322-cdna of hbfgf supplied by dr. seno (from molecular biology dept, okaido prefectural uni...

objective(s) lipocalin 2 (lcn2) is a 25-kda glycoprotein that has initially been extracted from neutrophil granules. expression of lcn2 is induced under various pathophysiological conditions. it is also known as an early marker of kidney and heart injury. high-level expression of recombinant lcn2 neutrophil gelatinase-associated(ngal) in insect cells was the aim of this study. materials and met...

background and objectives: urinary tract infection (uti) is one of the most common infections in the world. the majority of utis are caused by uropathogenic escherichia coli (upec) strains. fimh and flic are the most important virulence factors of upec. to date, any ideal vaccine against uti has not been approved for human use and we need to test new targets to develop an ideal vaccine against ...

objective: the aim of present study was cloning and expression of phic31 integrase cdna in a bacterial expression vector. thus, an intra molecular assay vector was applied to show in vitro activity of recombinant protein. materials and methods: in this experimental study, phic31 cdna was subcloned into a prokaryotic expression vector and transformed into e.coli bl21 (de3). recombinant phic31 in...

It is possible to use transgenic plants, as bioreactors, for the production of recombinant inexpensive chemicals and drug components. Transient gene expression is an appropriate alternative to stable transformation because it allows an inexpensive and rapid method for expression of recombinant proteins in plant tissues. In recent years, plant viral vectors have been increasingly developed as su...

automatic analysis of human facial expressions is one of the challenging problems in machine vision systems. it has many applications in human-computer interactions such as, social signal processing, social robots, deceit detection, interactive video and behavior monitoring. in this paper, we develop a new method for automatic facial expression recognition based on facial muscle anatomy and hum...