Stereological techniques using the optical disectors require estimation of final section thickness, but frozen tissue irregularities may interfere with this estimation. Cryostat slices from rodent nerve tissues (dorsal root ganglia, spinal cord, and brain), cut at 16, 40, and 50 μm, were digitized with a confocal microscope and visualized through 3D software. Geometric section thickness of tiss...

There are two common methods for preparing animal tissues for oxygen uptake studies; namely, the “slice technique” and the “mince technique.” In the former method the tissues are cut into thin slices about 0.3 mm. thick, care being taken to damage the tissues as little as possible. In the latter method no precautions are taken to avoid damage to the tissues, which are either run through a mecha...