Objective(s):Multi-epitopic protein vaccines and direction of vaccine delivery to dendritic cells (DCs) are promising approaches for enhancing immune responses against mutable pathogens. Escherichia coli is current host for expression of recombinant proteins, and it is important to optimize expression condition. The aim of this study was the optimization of multi-epitopic HIV-1 tat/pol/gag/env ...

We report on the measurement of saliva anti-Purified Protein Derivative sIgA and 38kDa antibodies from 127 children, of whom 31 were strong tuberculosis suspects and 96 were healthy contact children. The results concerning the percentage of children with antibody reactivity to PPD and 38kDa antigens showed that, of these 2 antigens, 38kDa induced higher reactivity in patients positive and negat...

Seventeen helper T-cell clones were derived by stimulating lymph node cells from sensitized C57BL/6 mice with Mycobacterium tuberculosis H37Ra, M. tuberculosis H37Rv, or purified protein derivative. Most clones cross-reacted with Mycobacterium bovis BCG, H37Ra, H37Rv, and purified protein derivative. However, four clones were able to differentiate H37Rv from H37Ra, or BCG from H37Ra and H37Rv. ...

Charles River mice were painted once with dimethylbenz(a)anthracene (tumor initiation), followed by repeated applications of croton oil (tumor promotion). The i.v. injection of Bacillus Calmette-Guérin(BCG) 3 weeks prior to the initiation of croton oil treatment reduced the incidence and rate of appearance of both benign and malignant skin tumors. After 50 applications of croton oil, (2/week f...

Purified protein derivative from Mycobacterium tuberculosis inhibits contact sensitivity to oxazolone in mice when given intravenously 24 to 72 h before the antigen. Transfer experiments showed that various types of suppressor cells occurred in the lymph nodes draining the site of sensitization: (i) anti-oxazolone idiotype + B lymphocytes, found at day 3 after sensitization, transferred suppres...

Objective(s)  Garlic (Allium sativum) is known as a potent spice and a medicine with broad therapeutic properties ranging from antibacterial to anticancer, and anticoagulant. One of the major purified garlic protein components is the 47 kDa protein. In this study, the effect of 47 kDa protein extracted from aged garlic (AGE) was evaluated on mouse dendritic cell (DC) maturation in vitro. Mate...

The PTB domain of mouse dok1 fusion protein has been overexpressed in Escherichia coli and crystallized in a form suitable for X-ray crystallographic study. Crystals have been obtained using the vapour-diffusion method and belong to space group P2(1)2(1)2(1). X-ray diffraction data were collected in-house to 2.5 A resolution. A selenomethionine (SeMet) dok1 PTB fusion-protein derivative was exp...