مهدی مهدوی
انستیتو پاستور ایران
[ 1 ] - تاثیر کمکی (اجوانتی) تمرین ورزشی استقامتی کوتاه مدت بر فعال‌سازی سلول‌های T تنظیمی در واکسن HSV-1 و یادآورهای آن
هدف: تاثیر فعالیت های ورزشی به صورت مزمن و حاد به عنوان ادجوانت واکسن در برخی از مطالعات بررسی شده است. هدف از پژوهش حاضر بررسی ادجوانتی یک دوره تمرین ورزشی استقامتی کوتاه مدت بر فعال‌سازی سلول‌های T تنظیمی در مدل واکسن HSV-1 بود. مواد و روش ها: 24 موش نژاد بالبسی در سه گروه کنترل، گروه واکسن و گروه دریافت کننده واکسن و تمرین ورزشی استقامتی کوتاه مدت تقسیم شدند. فعال سازی سلول‌های T تنظیمی پس ...
[ 2 ] - تأثیر شش هفته تمرین استقامتی بر سطوح برخی سایتوکاین‌های ریزمحیط تومور در موش‌های مادۀ مبتلا به تومور پستان
هدف از پژوهش حاضر بررسی تأثیرات تمرینات ورزشی بر تعادل سایتوکاینی موش‌های مادۀ مبتلا به سرطان پستان است. بدین منظور 20 سر موش بالبسی ماده تصادفی در دو گروه کنترل و تجربی قرار گرفتند. پس از آشناسازی با محیط و تزریق سلول‌های سرطانی به همۀ موش‌ها، گروه تجربی به مدت شش هفته، پنج روز و با شدت متوسط تمرینات استقامتی را انجام دادند. موش‌ها روزانه از نظر تغییرات رشد تومور ارزیابی شدند. در پایان موش‌ها...
[ 3 ] - تغییرات سایتوکاین‌های اینترلوکین ـ2 و اینترلوکین ـ12 در سلول‌های طحال موش‌های بالبسی به‌دنبال استفاده از یک وهله ورزش استقامتی به‌عنوان اجوانت واکسن ویروس هرپس سیمپلکس یک
در راستای افزایش کارایی واکسن‌ها، استفاده از اجوانت‌ها همواره موردتوجه ایمونولوژیست‌ها بوده است. فعالیت‌های ورزشی با توجه به تغییرات ایمونولوژیکی که ایجاد می‌کنند می‌توانند به‌عنوان اجوانت واکسن برای پاسخ‌های ایمنی مؤثر باشند. در این ارتباط، هدف از پژوهش حاضر بررسی اثر اجوانتی یک وهله ورزش استقامتی بر تولید سایتوکاین‌های اینترلوکین ـ2 و اینترلوکین ـ12 از سلول‌های طحال در مدل واکسن ویروس هرپس سی...
[ 4 ] - Development of monoclonal antibodies against axenic amastigotes of Leishmania infantum strain in Iran: Implication for diagnosis of Kala-azar
Objective(s): Leishmaniasis is endemic in 88 countries. Amastigote forms of Leishmania are experts at exploiting host cell processes to establish infection. Monoclonal antibodies are key reagents used in the diagnosis of infectious and non-infectious diseases. The aim of this study was to produce monoclonal antibodies against axenic amastigotes of the Leishmania infantum strain in Iran.Material...
[ 5 ] - Construction, expression, purification and characterization of secretin domain of PilQ and triple PilA-related disulfide loop peptides fusion protein from Pseudomonas aeruginosa
Objective(s): Infection with Pseudomonas aeruginosa has been a long-standing obstacle for clinical therapy due to the complexity of the genetics and pathogenesis, as well for widespread resistance to antibiotics, thus attaching great importance to explore effective vaccines for prevention and treatment. This paper focuses on the introduction of novel Pseudomonas aeruginosa type IV pili (T4P)-ba...
[ 6 ] - Potentiation of human papilloma vaccine candidate using naloxone/alum mixture as an adjuvant: increasing immunogenicity of HPV-16E7d vaccine
Objective(s): Many types of human papillomaviruses (HPVs) have been identified, with some leading to cancer and others to skin lesions such as anogenital warts. Studies have demonstrated an association between oncogenic HPV and cervical cancer and many researchers have focused on therapeutic vaccines development. At present, the modulatory effect of opioids on the innate and acquired immune sys...
[ 7 ] - Optimization of multi-epitopic HIV-1 recombinant protein expression in prokaryote system and conjugation to mouse DEC-205 monoclonal antibody: implication for in-vivo targeted delivery of dendritic cells
Objective(s):Multi-epitopic protein vaccines and direction of vaccine delivery to dendritic cells (DCs) are promising approaches for enhancing immune responses against mutable pathogens. Escherichia coli is current host for expression of recombinant proteins, and it is important to optimize expression condition. The aim of this study was the optimization of multi-epitopic HIV-1 tat/pol/gag/env ...
[ 8 ] - Effect of Invasive Aspergillosis Infection on the Immune Responses of Cancer Mice
Objective(s) Using a cancer murine model of invasive aspergillosis (IA), we investigated the expression of TLR-2, Dectin-1 and the level of cytokine production by CD4+ T helper cells in different groups of mice (with or without cancer), also, the effect of invasive aspergillosis on the immune response pattern of cancer mice. Materials and Methods Patterns of susceptibility and resistance to ...
[ 9 ] - تاثیر یک دوره تمرین مقاومتی شدید بر بیان پروتئین مایوکاین های IL-6،IL-1β و TNF-α در عضله تند تنش موش های صحرایی دیابتی
مقدمه: سایتوکاین ها و دیگر پپتیدهای بیان، تولید و آزاد شده به وسیله فیبرهای عضلانی که اثرات پاراکرین و اندوکرین اعمال می کنند به عنوان مایوکاین طبقه بندی می شوند. این احتمال وجود دارد که مایوکاین های آزاد شده از عضله اسکلتی واسطه اثرات مفید ورزش در رابطه با سلامتی باشند. هدف از مطالعه حاضر بررسی اثرات تمرین مقاومتی شدید بر بیان پروتئین مایوکاین های IL-6، IL-1β و TNF-α در عضله تند تنش در موش‌های...
[ 10 ] - Cloning, Expression and Purification of a Novel Multi-epitopic HIV-1 Vaccine Candidate: A Preliminary Study on Immunoreactivity
Introduction : Designing an effective vaccine against human immunodeficiency virus (HIV)-1 is a global health priority . Multi-epitope vaccines offer several potential advantages that may be promising in case of mutable divergent pathogens such as HIV-1. Herein, a multiepitopic recombinant protein containing various HIV-1 antigens was expressed in E. coli cells and its immunogenicity in combi...
[ 11 ] - تهیه نانوذره‌های poly lactic-co-glycolic acid (PLGA) حاوی آلژینات، لیپوپلی‌ساکارید و اگزوتوکسین A سودوموناس آئروژینوزا به‌عنوان نانو‌واکسن
مقدمه: باکتری سودوموناس آئروژینوزا به علت داشتن عوامل متعدد بیماری‌زایی و شیوع سویه‌های چند مقاومتی آن در سراسر دنیا اهمیت ویژه‌ای دارد و از این رو، نیاز به پیشگیری و تولید واکسن کارآمد برای آن ضروری است. هدف مطالعه حاضر، استفاده از نانوذره‌های poly lactic-co-glycolic acid (PLGA)در طراحی واکسن با آنتی‌ژن‌های‌آلژینات، لیپوپلی‌ساکارید و اگزوتوکسین A سودوموناس آئروژینوزا است. مواد و روش‌ها: در ...
[ 12 ] - Development of a Novel Anti-Adhesive Vaccine Against Pseudomonas aeruginosa Targeting the C-terminal Disulfide Loop of the Pilin Protein
The type IV pili (T4P) is a major virulence factor of Pseudomonas aeruginosa (P. aeruginosa) that is associated with primary adhesion, biofilm formation and twitching motility. This study focuses on the introduction of a novel biologically active subunit vaccine derived from the disulfide loop (DSL) of P. aeruginosa pilin. We investigated the expression of the novel PilA in-frame with pET26a ve...
[ 13 ] - High Yield Overexpression, Refolding, Purification and Characterization of Pseudomonas aeruginosa Type B-Flagellin: An Improved Method Without Sonication
Pseudomonas aeruginosa as an opportunistic pathogen is a significant cause of acute and chronic infections in patients with compromised defenses. This bacterium is motile via a single polar flagellum made of polymerized flagellin subunits differentiated into two major serotypes: A and B. flagellin plays an important role as a virulence factor in the adhesion, colonization and invasion of P. aer...
[ 14 ] - An HIV-1 Mini Vaccine Induced Long-lived Cellular and Humoral Immune Responses
Memory formation is the most important aspect of a vaccine which can guarantee long-lasting immunity and protection. The main aim of the present study was to evaluate the memory immune responses after immunization with a mini vaccine. Mice were immunized with human immunodeficiency virus-1 P24-Nef fusion peptide and then cellular and humoral immune responses were evaluated. In order to determin...
[ 15 ] - ساخت کتابخانه ژنی آنتی‌بادی ScFv انسانی بر پایه T Vector علیه توکسین کزاز
سابقه و هدف: امروزه آنتی‌بادی‌ها در حوزه‌های تشخیص و درمان جایگاه ویژه‌ای را به خود اختصاص داده‌اند. در این میان، آنتی‌بادی‌های مونوکلونال کاملاً انسانی به دلیل عدم برانگیختن پاسخ ایمنی در بدن و کارایی بالا در درمان بیماری‌ها مورد توجه قرار‌گرفته‌اند. تحقیق پیش‌رو به‌منظور ساخت کتابخانه ژنی آنتی‌بادی نوترکیب انسانی از یک فرد واکسینه با توکسوئید کزاز انجام گرفته است. مواد و روش‌ها: ابتدا از...
[ 16 ] - Th1 Immune Response Induction by Biogenic Selenium Nanoparticles in Mice with Breast Cancer: Preliminary Vaccine Model
Background: Tumor associated antigens can be viably used to enhance host immune response. Objectives: The immunomodulatory effect of biogenic selenium nanoparticles (SeNPs) was compared between treated and untreated mice with crude antigens of 4T1 cells. Materials and Methods: Female inbred BALB/c mice (60) were injected by cancinogenic 4T1 cells causing breast cancer. After 10 days, all tumor ...
[ 17 ] - بهینه‌سازی بیان سازه پلی‌توپیک حاوی ژن‌های E6 و E7 ویروس پاپیلومای انسانی در میزبان بیانی اشرشیاکلی
Background: Human papilloma virus is a DNA virus from the papillomavirus family that is most prevalent in human cervical cancers and many studies showed the E6 and E7 proteins are present in the majority of cervical cancer cases. Development of universal HPV peptide-based vaccine with more serotypes coverage has considerable value. The aim of the study was to design a multi-epitope universal va...
[ 18 ] - فرمولاسیون آنتی ژن HBs در ادجوانت MF59 و مقایسه ایمنی زایی آن با واکسن تجاری هپاتیت B
Introduction: Hepatitis is a systemic diseasethat causes liver inflammation. The prevention of this infection is a vaccination. The commonly used vaccine to fight this disease is to use the vaccine formulated with Alum. This vaccine cannot provide immune response and complete productivity in some people. In this study, cellular and humoral immune responses of hepatitis B vaccine were compared w...
[ 19 ] - Protective Potential of Conjugated P. aeruginosa LPS –PLGA Nanoparticles in Mice as a Nanovaccine
Background: Pseudomonas aeruginosa has an important role in nosocomial infections. Objective: To evaluate biological activity of the detoxified LPS (D-LPS) entrapped into Poly lactic-co-glycolic acid (PLGA) nanoparticles. Materials: LPS was extracted and detoxified from the P. aeruginosa strain PAO1. The D-LPS, conjugated to t...
[ 20 ] - Secreted Chemicals From Probiotic Bacteria Potentiate Th1 Pattern of Immune Cells and Apoptosis Induction in Breast Cancer and Gastric Adenocarcinoma Cell Lines
Cancer is one of the main causes of mortality. Therapeutic importance in some diseases, especially cancers has led to considering the anti-cancer effects of probiotics more than ever. Probiotics have stimulatory effects on the immunity and suppressor effects on cancer activity. Various studies report that probiotic bacteria have cytotoxicity effects on different types of solid tumors. In this r...
[ 21 ] - The Probiotic Bacteria Induce Apoptosis in Breast and Colon Cancer Cells: An Immunostimulatory Effect
Background: Uncontrolled cell proliferation and resistance to apoptosis are the main characteristics of cancer cells. Therefore, a substance with the capability to induce apoptosis in cancer cells could be known as an anti-cancer material. Probiotics are useful microorganisms that are crucial for the host’s health.Materials and Methods: In the present stu...
[ 22 ] - Immunopotentiotor Effect of α-Tocopherol on Cytokine Expression in the Lymphocytes in the Elderly People
Background: Aging is associated with attenuation of immune responses. Studies show that old people are vulnerable to infectious diseases such as influenza. α-Tocopherol as an immunomodulator affects immune responses. In the present study, the effect of α-tocopherol, on lymphocyte responses i.e. interferon-gamma (IFN-γ), Tumor Necrosis Factor-alpha (TNF-α), and nuclear factor-κB (NF-κB) in elde...
[ 23 ] - Assessment of Antitumor Activity of Vinca herbacea on Human Ovarian Cancer Cell Line
Background: It seems that Vinca. herbacea has an anti-tumor effect. Here, the immunotherapeutic effect of this compound is assessed against human ovarian cancer (SKOV3) cells because of the high incidence of this tumor in women. Materials and Methods: The cytotoxic activity of V. herbacea extract against human ovarian cancer (SKOV3) cells was determined by MTT assay. The apoptosis-inducing pote...
[ 24 ] - Lactobacillus Acidophilus Cytotoxicity Effect and Apoptosis in Human Bladder Carcinoma Cells: An In Vitro Study
Introduction: Anticancer effects of Lactobacillus acidophilus, as a probiotic bacterium, have been indicated in several studies. There are common therapeutic options for bladder cancer treatment; however, their side effects and recurrence of disease are considerable. Therefore, complementary medication is essential and it must be safe and effective. In the present research, we assessed the anti...
[ 25 ] - Evaluation of humoral and cellular immunity of recombinant autolysin protein Staphylococcus aureus in mouse model
Background: Staphylococcal aureus is a gram positive cocci and opportunistic pathogen. Due to the spread of this bacterium to antibiotics resistance, one of the most important ways of prevention is the use of vaccines. In this respect, autolysin protein as one of the adhesion molecule of bacteria plays an important role for binding bacteria to the host cells and cell division. Herein, the role ...
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