a collection of enterococci  spp. (about 96 isolates) were isolated from two iranian raw milk cheeses, namely lighvan and koozeh, and subsequently, identified as enterococcus faecium , enterococcus faecalis , enterococcus durans , enterococcus casseliflavus and enterococcus italicus by 16s rdna sequencing. all of the 96 isolates were subjected to agar-spot and well-diffusion assay in order to d...

The rep-PCR DNA fingerprint technique, which uses repetitive intergenic DNA sequences, was investigated as a way to differentiate between human and animal sources of fecal pollution. BOX and REP primers were used to generate DNA fingerprints from Escherichia coli strains isolated from human and animal sources (geese, ducks, cows, pigs, chickens, and sheep). Our initial studies revealed that the...

Adeno-associated virus type 2 (AAV) is known to establish latency by preferential integration in human chromosome 19q13.42. The AAV non-structural protein Rep appears to target a site called AAVS1 by simultaneously binding to Rep-binding sites (RBS) present on the AAV genome and within AAVS1. In the absence of Rep, as is the case with AAV vectors, chromosomal integration is rare and random. For...

The Lactobacillus plantarum and pentosus genotypes existing in industrial-scale cucumber fermentations were defined using rep-PCR-(GTG)5. ability of each genotype to ferment cucumbers under various conditions was evaluated. Rep-PCR-(GTG)5 the technique capable illustrating most intraspecies discrimination compared sequencing housekeeping genes (recA, dnaK, pheS rpoA), MLST RAPD with primers LP1...

tan spot disease of wheat with the causal agent of pyrenophora tritici-repentis is a very serious  disease throughout the world causing severe yield losses. out of 163 isolates of the pathogen, collected from golestan and mazandaran provinces, 58 were selected for a study of population genetic structure. selecting the real representatives from the whole population was carried out according to t...

Bulking of activated sludge is a world-wide problem which negatively affects wastewater treatment efficiency. The most common reasons of bulking are bacterial community changes, especially excessive growth of filamentous bacteria (filamentous bulking) or excess of biopolymers on the surface of non-filamentous microbes (non-filamentous or Zoogleal bulking). Because of the complex nature of the b...