Introduction: The objective of the present research was to determine the effect of low-intensity eccentric training with blood flow restriction (BFR) on Hepatocyte Growth Factor (HGF) and myogenic factor 5 (Myf5), in non-athletes young men. Methods: In this study, 20 non-athlete young men were allocated to two eccentric training and eccentric training with BFR groups. Eccentric training progra...

Signaling through the mammalian target of rapamycin (mTOR) in response to leucine modulates many cellular and developmental processes. However, in the context of satellite cell proliferation and differentiation, the role of leucine and mTORC1 is less known. This study investigates the role of leucine in the process of proliferation and differentiation of primary preterm rat satellite cells, and...

In the present study, we examined the roles of hepatocyte growth factor (HGF) and nitric oxide (NO) in the activation of satellite cells in passively stretched rat skeletal muscle. A hindlimb suspension model was developed in which the vastus, adductor, and gracilis muscles were subjected to stretch for 1 h. Satellite cells were activated by stretch determined on the basis of 5-bromo-2'-deoxyur...

BACKGROUND Preterm skeletal muscle genesis is a paradigm for myogenesis. The role of mitogen-activating protein kinase kinase kinase kinase-3 (MAP4K3) in preterm skeletal muscle satellite cells myogenesis or its relationship to mammalian target of rapamycin complex 1 (mTORC1) activity have not been previously elaborated. MATERIAL AND METHODS Small interfering RNA (siRNA) interference technology...

Skeletal muscle is a striated tissue composed of multinucleated fibers that contract under the control of the somatic nervous system to direct movement. The stem cells of skeletal muscle, known as satellite cells, are responsible for muscle fiber growth, turnover, and regeneration. Satellite cells are activated and proliferate in response to stimuli, and simplistically, have two main fates-to r...

The satellite cell (6) is a mononucleated cell that is wedged between the multinucleated muscle syncytium and the basal membrane of the myofiber. These cells are described in skeletal muscle of amphibians (6, 7) and mammals (3, 12-14). From data in the literature it is clear that satellite cells are most abundant during embryonic life, though it is not obvious that they participate in the norma...

Satellite cells are myogenic stem cells responsible for the post-natal growth, repair and maintenance of skeletal muscle. This review focuses on the basic biology of the satellite cell with emphasis on its role in muscle repair and parallels between embryonic myogenesis and muscle regeneration. Recent advances have altered the long-standing view of the satellite cell as a committed myogenic ste...

Satellite cells are mitotically quiescent myogenic stem cells resident beneath the basal lamina surrounding adult muscle myofibers. In response to injury, multiple extrinsic signals drive the entry of satellite cells into the cell cycle and then to proliferation, differentiation, and self-renewal of their downstream progeny. Because satellite cells must endure for a lifetime, their cell cycle a...

The dystrophin-associated glycoprotein complex (DGC) is found at the muscle fiber sarcolemma and forms an essential structural link between the basal lamina and internal cytoskeleton. In a set of muscular dystrophies known as the dystroglycanopathies, hypoglycosylation of the DGC component α-dystroglycan results in reduced binding to basal lamina components, a loss in structural stability, and ...

Skeletal muscle development in avian and mammalian embryos depends on the proliferation, differentiation, and fusion of embryonic myoblasts. During the late fetal period and following birth or hatching, myogenic satellite cells are responsible for this developmental function. Satellite cells, which are found adjacent to existing skeletal muscle fibers fuse with these fibers and their nuclei dir...