introduction: satellite cells are known as the main regenerative cell type in skeletal muscles. our study established a modified digestion and preplating method for the isolation of slow or weak adherent cells for the enrichment of satellite cells. low-survival rate of these primary stem cells prompted us to address whether cell culture medium substitution might change cell viability status. me...

Introduction: Satellite cells are known as the main regenerative cell type in skeletal muscles. Our study established a modified digestion and preplating method for the isolation of slow or weak adherent cells for the enrichment of satellite cells. Low-survival rate of these primary stem cells prompted us to address whether cell culture medium substitution might change cell viability status. M...

the aim of this study was to investigate the effect of 8 weeks of selective exercise activity and estrogen supplement on satellite cells in ovariectomized sprague-dawley rats. at first, subjects were ovariectomized and after one month of complete recovery, they were randomly divided into four groups: control, exercise activity, estrogen supplement, and exercise and estrogen supplement. only exe...

satellite cells (scs) are the most abundant skeletal muscle stem cells. they are widely recognized for their contributions to maintenance of muscle mass, regeneration and hypertrophy during the human life span. these cells are good candidates for cell therapy due to their self-renewal capabilities and presence in an undifferentiated form. presently, a significant gap exists between our knowledg...

Duchenne muscular dystrophy (DMD) is a devastating disease characterized by muscle wasting, loss of mobility and death in early adulthood. Satellite cells are muscle-resident stem cells responsible for the repair and regeneration of damaged muscles. One pathological feature of DMD is the progressive depletion of satellite cells, leading to the failure of muscle repair. Here, we attempted to exp...

Satellite cells are the resident stem cells of adult skeletal muscle. To date though, there is a paucity of native markers that can be used to easily identify quiescent satellite cells, with Pax7 probably being the best that is currently available. Here we have further characterized a number of recently described satellite cell markers, and also describe novel ones. Caveolin-1, integrin alpha7 ...

Adult skeletal muscle possesses extraordinary regeneration capacities. After muscle injury or exercise, large numbers of newly formed muscle fibers are generated within a week as a result of expansion and differentiation of a self-renewing pool of muscle stem cells termed muscle satellite cells. Normally, satellite cells are mitotically quiescent and reside beneath the basal lamina of muscle fi...

Skeletal muscle satellite cells are a muscle stem cell population that mediate posthatch muscle growth and repair. Satellite cells respond differentially to environmental stimuli based upon their fiber-type of origin. The objective of this study was to determine how temperatures below and above the in vitro control of 38°C affected the proliferation and differentiation of satellite cells isolat...

Satellite cells play a central role in mediating the growth and regeneration of skeletal muscle. However, whether satellite cells are stem cells, committed progenitors, or dedifferentiated myoblasts has remained unclear. Using Myf5-Cre and ROSA26-YFP Cre-reporter alleles, we observed that in vivo 10% of sublaminar Pax7-expressing satellite cells have never expressed Myf5. Moreover, we found tha...

Satellite cells are responsible for growth, maintenance and repair of postnatal skeletal muscle. On the search for a specific marker molecule for the identification of satellite cells by light-microscopy, we examined M-cadherin, a Ca-dependent cell adhesion molecule which is present in quiescent as well as activated satellite cells but not in other cell types in skeletal muscle. To determine th...