نتایج جستجو برای: scnt embryos
تعداد نتایج: 43864 فیلتر نتایج به سال:
Pancreatic and duodenal homeobox 1 (PDX1) plays a crucial role in pancreas development, β-cell differentiation, and maintenance of mature β-cell function. In this study, we designed a strategy to produce PDX1-knockout (KO) pigs. A transcription activator-like effector nuclease (TALEN) pair targeting exon 1 of the swine PDX1 gene was constructed. Porcine fetal fibroblasts (PFFs) were transfected...
Somatic cell nuclear transfer (SCNT) is a reproductive biotechnology with great potential in the reproduction of different species zootechnical interest, including sheep. This study aimed to carry out bibliometric analysis scientific papers published on application SCNT sheep during period 1997–2023. The search involved Science Citation Index Expanded and Social Sciences databases main collecti...
The following experiments compared the efficiency of three fusion/activation protocols following somatic cell nuclear transfer (SCNT) with porcine somatic cells transfected with enhanced green fluorescent protein driven by the chicken beta-actin/rabbit beta-globin hybrid promoter (pCAGG-EGFP). The three protocols included electrical fusion/activation (NT1), electrical fusion/activation followed...
Background: The oocyte is a unique cell committed to reprogram fertilizing sperm and to support early stages of embryonic development until the species-specific stage of zygote genome activation that occurs around the second to third cell cycle in sheep embryos. In this sense, considering the huge list of oocyte transcripts, we selected some candidates genes based on their roles of regulating di...
Somatic cell nuclear transfer (SCNT) is an assisted reproductive technique that can produce multiple copies of excellent livestock. However, low cloning efficiency limits the application of SCNT. In this study, we systematically investigated the major influencing factors related to the overall cloning efficiency in pigs. Here, 13620 cloned embryos derived from excellent pigs were transferred in...
Background: The potential applications of a simplified method of somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput is considerable. Technically, a major step of SCNT is to produce large pools of enucleated oocytes (cytoplasts) efficiently, a process that requires considerable micromanipulation skill and expensive equipment. Here, we have devel oped an efficient...
Some human embryonic stem cell lines have shown genomic instabilities over long-term culture. To study the controversial origin of the SCNT-hES-1 line, which was derived from autologous somatic cell nuclear transfer (SCNT), we compared the expression and methylation patterns of imprinted genes in the SCNT-hES-1 cells with the donor's somatic cells by semi-quantitative RT-PCR, real-time PCR and ...
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