BACKGROUND Current routine semen preparation techniques do not inclusively target all intrinsic sperm characteristics that may impact the fertilization potential. In order to address these characteristics, several methods have been recently developed and applied to sperm selection. The objective of this study was to systematically review the literature describing these advanced sperm selection ...

As a source of growth factors, platelet-rich plasma (PRP) has been widely used in the repair various injuries due to its cytoprotective properties regenerative medicine. The objective present study was investigate effect autologous PRP supplementation on quality frozen-thawed human sperm. Twelve normozoospermic semen samples were collected, and each sample divided into 4 aliquots added with dif...

Sperm DNA integrity is essential for accurate transmission of genetic material to offspring. Fragmentation of genomic DNA is an initial hallmark of apoptosis (programmed cell death). The aim of this study was to determine sperm nuclear DNA integrity and mitochondrial function, to quantify possible apoptosis and to investigate any relationship between these parameters. Semen samples (n = 25) wer...

INTRODUCTION The selection of a sperm with good genomic integrity is an important consideration for improving intracytoplasmic sperm injection (ICSI) outcome. Current convention selects sperm by vigour and morphology, but preliminary evidence suggests selection based on hyaluronic acid binding may be beneficial. The aim of the Hyaluronic Acid Binding Sperm Selection (HABSelect) trial is to dete...

Background: Cisplatin (CIS), an anticancer alkylating agent, induces DNA adducts and effectively cross links the DNA strands and so affects spermatozoa as a male reproductive tract toxicant. Materials and Methods: The present study investigated the cellular/biochemical mechanisms underlying possible protective effect of selenium nano-particles (Nano-Se) as an established strong antioxidant with...

Sperm concentration, morphology, DNA condensation and nuclear protein pattern as well as sperm adenylate cyclase were evaluated in semen samples provided by 7 volunteers every 8 h for 2 days. During the observation period, sperm concentration and total sperm number decreased but began to return towards normal after a 3-day abstinence period. No increase in the proportion of immature sperm cells...

OBJECTIVE This study evaluated the integrity of the chromatin structure, reactive oxygen species (ROS) levels, mitochondrial membrane potential (MMP), DNA damage and lipid peroxidation of semen samples from infertile men classified as unexplained infertility. METHODS Between February 2010 and July 2011 semen parameters and functional tests were evaluated in 10 subjects with proven fertility, ...

background: exposure to arsenic is associated with impairment of male reproductive function by inducing oxidative stress. silymarin with an antioxidant property scavenges free radicals. objective: the aim of this study was to investigate if silymarin can prevent the adverse effects of sodium arsenite on ram sperm plasma membrane and acrosome integrity. materials and methods: ram epidydimal sper...

AIM To compare the seminal attributes of neat, pre-freeze (at equilibration), and post-freeze (24 h after freezing) semen in pure and crossbred Jersey bulls. MATERIALS AND METHODS Total 36 ejaculates (3 ejaculates from each bull) were collected from 6 pure Jersey and 6 crossbred Jersey bulls and evaluated for various seminal attributes during neat, pre-freeze, and post-freeze semen. RESULTS...

Genetic and biochemical sperm integrity is essential to ensure the reproductive competence. However, spermatogenesis involves physiological changes that could endanger sperm integrity. DNA protamination and apoptosis have been studied extensively. Furthermore, elevated rates of aneuploidy and DNA injury correlate with reproductive failures. Consequently, this study applied the conventional sper...