The synthesis of amylose in amyloplasts is catalyzed by granule-bound starch synthase (GBSS). GBSS gene expression was inhibited via antisense RNA in Agrobacterium rhizogenes-transformed potato plants. Analysis of starch production and starch granule composition in transgenic tubers revealed that reduction of GBSS activity always resulted in a reduction of the production of amylose. Field exper...

A field experiment was conducted with four wheat varieties Raj4134, HD2733, NW 1014 and K9006 for characterization of heat stress tolerance traits under heat stress condition. Heat stress was induced by delayed sowing of 35 days from normal date of sowing so that grain filling stage of wheat could experience severe heat stress. Data related to plant height, chlorophyll content, starch content a...

Hydrogen and oxygen isotopic fractionation relative to medium water for two different carbohydrate metabolic pathways leading to cellulose synthesis were measured. This was accomplished by analysing stable hydrogen and oxygen isotope ratios of water and cellulose for seedlings. The seedlings had been germinated and heterotrophically grown in closed vessels from species having starch (Triticum a...

Over 40,000 species of plants accumulate fructan, [beta]-2-1- and [beta]-2-6-linked polymers of fructose as a storage reserve. Due to their high fructose content, several commercial applications for fructans have been proposed. However, plants that accumulate these polymers are not agronomically suited for large-scale cultivation or processing. This study describes the transformation of a Bacil...

Isolation and identification of thermophilic Bacillus sp was carried out from a soil sample. The cells were cultivated in a medium containing soluble starch as sole carbon source. The addition of calcium (10 mM) or peptone (1%) and yeast extract (0.5%) to the medium shortened the lag period and improved the growth and amylase synthesis. The optimum temperature for amylase production was detecte...

Many eukaryotic microalgae modify their metabolism in response to nutrient stresses such as phosphorus (P) starvation, which substantially induces storage metabolite biosynthesis, but the genetic mechanisms regulating this response are poorly understood. Here, we show that P starvation-induced lipid and starch accumulation is inhibited in a Chlamydomonas reinhardtii mutant lacking the transcrip...

Waxy wheat (Triticum aestivum L.) lacks the waxy protein, which is also known as granule-bound starch synthase I (GBSSI). The starch granules of waxy wheat endosperm and pollen do not contain amylose and therefore stain red-brown with iodine. However, we observed that starch from pericarp tissue of waxy wheat stained blue-black and contained amylose. Significantly higher starch synthase activit...

In the present study, the effects of partial substitution of dietary protein by digestible starch on endogenous glucose production were evaluated in European seabass (Dicentrarchus labrax). The fractional contribution of dietary carbohydrates v. gluconeogenesis to blood glucose appearance and hepatic glycogen synthesis was quantified in two groups of seabass fed with a diet containing 30% diges...

While the beneficial physical properties of silicone polymers are exploited in many sustainable applications, high energy requirement for their synthesis compromises to a degree sustainability. We report strategy mitigate this issue by filling with inexpensive and renewable starch. Elastomeric materials covalently grafted starch, utilizing anhydride-modified silicones, permits loading up about ...