This paper discuss about a novel technique of developing a nano-structured polypyrrole biosensor for measurement of very low concentrations of hydrogen peroxide in liquid media. The proposed fabrication method is very effective in growing a nano-structured conducting polymer layer on a planar conducting substrate. In addition enzyme loading was done under a high electric field of 1000V/m. The d...

The formation of hydrogen peroxide during the oxidation of NADH by purified preparations of cytochrome o has been demonstrated by employing three independent methods: polarographic, calorimetric, and fluorometric. The first two methods were used to assay for the accumulation of hydrogen peroxide and showed that hydrogen peroxide did accumulate as a product, but only about 30% of the oxygen cons...

background: high amount of heavy metals in sludge is one of the major obstacles to its use on farms. the present study aimed to investigate the possibility of leaching heavy metals from wastewater sludge by fenton method and determine the optimum level of parameters, such as iron, hydrogen peroxide, time, and ph for fenton reaction.   methods: the effects of various parameters, such as ph (2-9)...

BACKGROUND Because the vascular endothelium is exposed to oxidant stress resulting from ischemia/reperfusion and from the products of polymorphonuclear leukocytes or monocytes, studies were performed to examine the effect of hydrogen peroxide (1 micromol/L to 10 mmol/L) on endothelial Ca2+ signaling. METHODS AND RESULTS At low concentrations (1 to 10 micromol/L), hydrogen peroxide did not aff...

The oxidation of aqueous solutions carbamazepine is conducted using the Fenton reagent, combined with photolytic action a 150 W medium pressure UV lamp, operating at T = 40 °C. effect acidity analysed an interval pH 2.0–5.0, verifying that 5.0 promotes colour formation (Colour 0.15 AU). iron studied, finding water increases in linear way, Colour 0.05 + 0.0075 [Fe]0. oxidising hydrogen peroxide ...

Alpha-ketoglutarate, a key intermediate in the Krebs cycle, has been reported to benefit intestinal health. We tested whether alpha-ketoglutarate can protect intestinal cells against hydrogen peroxide induced damage and aimed to reveal the underlying mechanism. Intestinal porcine epithelial cell line J2 were cultured in Dulbecco's Modified Eagle Medium-High glucose with or without alpha-ketoglu...

Background: Ornithine (Orn) plays an essential role in the metabolism of plant cells through incorporation in polyamines biosynthesis, the urea cycle and nitrogen metabolism. Physiological response of the plant cells to its two enantiomers have not been widely investigated yet.Objectives: This study aimed to evaluate effect of ornithine enantiomers on exp...