Abstract Our previous studies showed that various extracts of Persian shallot (Allium hirtifolium) have anti-angiogenic effects. This study has been undertaken to isolate and identify the major effective anti-angiogeneic sub-fraction of shallot. After preparation of the 50% hydroalcoholic extract of shallot bulbs, the extract was successively fractionated into n-hexane, ethyl acetate, n-butanol...

Background: Holothuroids (sea cucumbers) are members of the phylum echinodermata, which produce saponins. Saponins exhibit a wide spectrum of pharmacological and biological activities. In this study, we isolated the crude saponins from the body wall of the dominant Iranian species of sea cucumber, Holothuria leucospilota (H. leucospilota). The purpose of this study was to confirm the presence o...

In this work, two chromatographic methods have been developed and validated for the determination of sertindole (an antipsychotic agent) in the presence of its oxidative degradation product. Sertindole was subjected to stress stability studies, including acid, alkali, oxidative, photolytic and thermal degradation. The chromatographic methods included the use of thin-layer chromatography (TLC-de...

Aflatoxins, zearalenone, deoxynivalenol, fumonisins, and their respective metabolites require specific procedures for their determination because of their diverse chemistry and occurrence in complex matrices of feedstuffs and foods. Major sources of error in the analysis of these mycotoxins arise from inadequate sampling and inefficient extraction and cleanup procedures. The determinative step ...

BACKGROUND AND AIMS The emergence of novel diseases caused by microbial pathogens and the undesirable side effects of certain antibiotics has been a recent dilemma in the medical arena. Consequently, it has stirred the discovery of many naturally occurring agents which could possibly provide important ramifications against various pharmacological targets and to combat various ailments. The main...

Plastoglobules are plastid compartments designed for the storage of neutral lipids. They share physical and structural characteristics with cytosolic lipid droplets. Hence, special care must be taken to avoid contamination by droplets during plastoglobule purification. We describe isolation pure plastoglobules from Arabidopsis thaliana leaves, methods we use determine their composition. After p...

Pressurized planar electrochromatography (PPEC) is a separating technique in which an electric field is applied to force the mobile phase movement through a porous media (electroosmotic effect). High separation efficiency, fast separations and changes in separation selectivity in comparison to liquid chromatography, especially thin layer chromatography (planar chromatography, TLC), are features...

Authors discuss problems with identification and determination of flunitrazepam and zolpidem in biological material (BM). Over the recent years, these two structurally different substances have become the most frequently used as well as abused hypnotic drugs. This study presents applicability of immunochemical methods in the screening of flunitrazepam, one of the most commonly prescribed drugs ...

phy 665: 243. Junk GA, Richard JJ, Grieser MD et al. (1974) Use of Macroreticular resins in the analysis of water for trace organic contaminants. Journal of Chromatography 99: 745. Puig D and BarceloH D (1995) Off-line and on-line solidphase extraction followed by liquid chromatography for the determination of priority phenols in natural waters. Chromatographia 40: 435. Puig D and BarceloH D (1...

Synthetic dyes comprise a large group of organic, organic salt and organometallic compounds which number in the thousands. Most individual dyes are assigned colour index (CI) numbers which helps to identify structure and properties as well as identity where a series of names have been used by the manufacturers for the same dye. Pigments are also listed and can be either organic or inorganic in ...