Recent theory has suggested that dosage compensation mediates sexual antagonism over X-linked genes. This process relies on the assumption scales phenotypic effects between sexes, which is largely untested. We evaluated this by quantifying transcriptome variation associated with a recently arisen, male-beneficial, mutation across tissues of field cricket Teleogryllus oceanicus , and testing rel...

background: in this study, we investigated the prevalence of staphylococcus aureus agr groups to detect the predominant type according to the source of isolation and assessed the possible relationship between agr groups, types of infection and susceptible or resistance to methicillin. materials and methods: dna of 194 s. aureus isolates were extracted by lysozyme-phenol chloroform method that i...

Selection of relevant genes for sample classification is a common task in most gene expression studies. As a powerful classification approach, random forest has been applied in this field, and it shows excellent performance compared with other classification methods. The measure of variable importance is the key of gene selection using random forest. However, the existing methods just consider ...

Neisseria meningitidis is the most common cause of meningitis and causes epidemic outbreaks. One trait of N. meningitidis, which is associated with most of the currently recognized virulence determinants, is the presence of phase-variable genes that are suspected to enhance its ability to cause an invasive disease. To detect the immune responses to phase-variable expressed proteins, we applied ...

Many host-adapted bacterial pathogens contain DNA methyltransferases (mod genes) that are subject to phase-variable expression (high-frequency reversible ON/OFF switching of gene expression). In Haemophilus influenzae and pathogenic Neisseria, the random switching of the modA gene, associated with a phase-variable type III restriction modification (R-M) system, controls expression of a phase-va...

Antibody repertoires for library construction are conventionally harvested from mRNAs of immune cells. To examine whether germline rearranged immunoglobulin (Ig) variable region genes could be used as source of antibody repertoire, an immunized phage-displayed scFv library was prepared using splenocytic genomic DNA as template. In addition, a novel frame-shifting PCR (fsPCR) step was introduced...

introduction ventilator-associated pneumonia (vap)  is one of the most serious and  prevalence of  complication  among patients in the intensive care unit nosocomial infections, which are often recognized after detecting symptoms. to date, there has been no proper clinical and diagnostic marker for early detection of this disease. in this study, two hcn4 and adam8 genes in patients with vap wer...

the quality traits of triticum dicoccoides ku-13441 (triticum turgidum l. var. dicoccoides (aabb, 2n= 4x= 28)) were analyzed by mixograph, and the results showed that t. dicoccoides ku-13441 had desirable qualities in gluten strength and flour stirring tolerance. subsequently, seventeen novel full-orf α-gliadin genes and thirty-five pseudogenes were cloned and sequenced from t. dicoccoides ku-1...