IA-2 (islet cell Ag 512) and IA-2 beta (phogrin/IAR) are related autoantigens associated with type 1 diabetes. To determine the critical regions for autoantibody binding and which of these autoantigens is the primary target, mutant and chimeric constructs were used to characterize Ab epitope binding in sera from 217 new onset patients with type 1 diabetes and sequential samples from 141 islet c...

IA-1 is a novel cDNA originally isolated from a human insulinoma subtraction library (ISL-153). It encodes a protein containing both a zinc finger DNA-binding domain and a putative prohormone domain. IA-1 transcripts have been found thus far only in tumors of neuroendocrine origin. Clinical studies have shown that IA-1 is a sensitive marker for neuroendocrine differentiation of human lung tumor...

Background and Purpose: Invasive aspergillosis (IA) is one of the most common life-threatening fungal infections among the critically ill patients including intensive care unit (ICU) patients. Delayed diagnosis and therapy may lead to poor outcomes. Diagnosis may be facilitated by a test for molecular biomarkers, i.e. detection of galactomannan (GM) antigen based on enzyme immunoassay, which is...

The I region of the mouse H-2 complex is divided into subregions, I-A, I-B, I-J, I-E, and I-C, that are marked by the Ia-1, Ia-2, Ia-4, Ia-5, and Ia-3 loci, respectively (1, 2). The Ia-1, Ia-5, and Ia-3 locus structural products are Ia antigens, a group of polymorphic, cell-surface glycoproteins that consist of two noncovalently associated polypeptide chains of mol wt =30,000-35,000 (a-subunit)...

The monoclonal antibodies (mAb) DA6.147, DA6.164, and HIG.48 against human Ia antigens, but not the W6/32 mAb against human class I major histocompatibility complex antigens or the anti-monocyte OKM1 and 63D3 mAb, stimulated monocytes to secrete interleukin 1 (IL-1). IL-1 was measured by its property of promoting the production of interleukin 2 (IL-2) by phytohemagglutinin-treated LBRM-33 clone...

The I region of the mouse H-2 complex is divided into subregions, I-A, I-B, I-J, I-E, and I-C, that are marked by the Ia-1, Ia-2, Ia-4, Ia-5, and Ia-3 loci, respectively (1, 2). The Ia-1, Ia-5, and Ia-3 locus structural products are Ia antigens, a group of polymorphic, cell-surface glycoproteins that consist of two noncovalently associated polypeptide chains of mol wt =30,000-35,000 (a-subunit)...

BACKGROUND HIV/AIDS pandemic brought into focus the importance of safe blood donor pool. AIMS To analyze true seroprevalence of HIV infection in our blood donors and devise an algorithm for donor recall avoiding unnecessary referrals to voluntary counseling and testing centre (VCTC). MATERIALS AND METHODS 39,784 blood units were screened for anti-HIV 1/2 using ELISA immunoassay (IA-1). Samp...