Introduction: Helicobacter pylori (H. pylori), is a bacterium responsible for upper gastrointestinal tract diseases. The 16s rRNA is a common H. pylori gene which are usually preferred for diagnosis purpose. The aim of this study was to determine the prevalence and abundance of 16s rRNA in fecal samples and also evaluate correlation between the level of 16s rRNA and activities of the cytokine...

objective: toxoplasmosis is a worldwide disease, for which different detection methods have been used. the nucleic acid sequence-based amplification (nasba) method is shown to be highly efficient for diagnosis of live microorganisms. the present research evaluates the molecular isothermal method of nasba to identify live toxoplasma gondii (t. gondii) in rat. methods: tachyzoites of t. gondii w...

objective :cryptosporidiosis is one of the most important parasitic infections in iran which causes diarrhea in humans and animals. the identification of the cryptosporidium species among humans is necessary. this study aims to identify species of cryptosporidium isolated from patients that referred to three hospitals in tehran based on the 18s rrna gene by nested pcr-rflp assay. ‎methods :in t...

background: clarithromycin resistance in helicbacter pylori has been found to be associated with point mutations in 23s rrna gene leads to reduced affinity of the antibiotic to its ribosomal target or changing the site of methylation. the aim of this study was to determine the most important point mutations in 23s rrna gene in h. pylori that are closely related to clarith-romycin resistance amo...

cryptosporidium species are one of the most common causes of gastrointestinal infection in humans around the world. this study has aimed to investigate the hyper variable region of the 18s rrna gene in cryptosporidium for exact parasite identification . dna was extracted from 26 fecal samples from which initially cryptosporidium oocysts were identified by ziehl-neelsen acid-fast , auramine phen...

  Cryptosporidium species are one of the most common causes of gastrointestinal infection in humans around the world. This study has aimed to investigate the hyper variable region of the 18S rRNA gene in Cryptosporidium for exact parasite identification .   DNA was extracted from 26 fecal samples from which initially Cryptosporidium oocysts were identified by Ziehl-Neelsen acid-fast , Auramine ...

introduction: helicobacter pylori (h. pylori), is a bacterium responsible for upper gastrointestinal tract diseases. the 16s rrna is a common h. pylori gene which are usually preferred for diagnosis purpose. the aim of this study was to determine the prevalence and abundance of 16s rrna in fecal samples and also evaluate correlation between the level of 16s rrna and activities of the cytokines,...

background: there is a conserved portion in the 16s rrna gene of bacteria which can be amplified by the universal pcr method. this fragment is 996 bp in length. in this method, only one set of universal primers is used for the amplification of the conserved region of the 16s rrna gene, in common bacterial pathogens. therefore, using the universal pcr method, these bacteria are detectable only b...

background: bovine theileriosis results from infection with obligate intracellular protozoa of the genus theileria . the phylogenetic relationships between two isolates of theileria annulata , and 36 theile­ria spp . , as well as 6 outgroup including babesia spp . and coccidian protozoa were analyzed using the 18s rrna gene sequence. methods: the target dna segment was amplified by pcr. the pcr...