This study reports the purification and biochemical characterization of thermostable and acidic-pH-stable α-amylase from Bacillus sp. Iranian S1 isolated from the desert soil (Gandom-e-Beryan in Lut desert, Iran). Amylase production was found to be growth associated. Maximum enzyme production was in exponential phase with activity 2.93 U ml-1 at 50°C and pH 5. The enzyme was purified by isoprop...

Pre-harvest sprouting (PHS) is one of the most important factors to affect the yield and quality of crops worldwide especially in wet harvest period. Breeding PHS-resistant cultivars has important implications to improve the wheat quality and production. The PHS is determined by environmental conditions, inner factors and interaction between these factors. We here reviewed recent advances influ...

Use of biosurfactants as a green stabilizer for the biological synthesis of gold nanoparticles (AuNPs) is now emerging as nontoxic and environmentally acceptable "green chemistry" procedures. Stability of AuNPs at different pHs is very important because our body has different pHs. This paper addresses this issue. In this work, first P. aeruginosa PTCC 13401 was used to produce rhamnolipid biosu...

besides the high starch content, sweet potato contains endogenous amylases where the two predominants are - and β-amylases. sweet potato is thought to be a promising source of β -amylase since β -amylase is one of the major protein in the tubers. this investigation has focused on the properties of β amylase from white-flesh sweet potato that has been grown in iran as a potential source for indu...

An extremely haloalkaliphilic archaeon Natrinema sp. SSBJUP-1 showing potent amylolytic and proteolytic activities was isolated from the saline alkaline Lonar Lake, India. Amylase and protease were active over the neutral to alkaline pH range from 6 to 9. Amylase was more stable over a narrower temperature range of 40-60C than the protease but had the higher optimum (55C) for activity. By contr...

Besides the high starch content, sweet potato contains endogenous amylases where the two Predominants are - and β-amylases. Sweet potato is thought to be a promising source of β -amylase since β -amylase is one of the major protein in the tubers. This investigation has focused on the properties of β amylase from white-flesh sweet potato that has been grown in Iran as a potential source for indu...

Ten bacterial strains isolated from the soil samples in the presence of cyclohexane were screened for amylase production. Among them, culture RG-01 was adjudged as the best amylase producer and was identified as Bacillus tequilensis from MTCC, Chandigarh. The isolate showed maximum amylase production (8100 U/mL) in the presence of starch, peptone, and Ca(2+) ions at 55°C pH 7.0 within 24 h of i...

Preharvest sprouting (PHS), the germination of grain on the mother plant under cool and wet conditions, is a recurring problem for wheat farmers worldwide. α-amylase enzyme produced during PHS degrades starch resulting in baked good with poor end-use quality. The Hagberg-Perten Falling Number (FN) test is used to measure this problem in the wheat industry, and determines how much a farmer's whe...