BACKGROUND In direct techniques of flow cytometry, the optimal antibody dilution or titer point is established from the plateau area of the antibody titration curve. However, the plateau area is defined without any statistical criteria, which may lead to an incorrect selection of antibody dilution. Herein, we report statistical criteria to establish the optimal antibody dilution for CD14, CD8, ...

the aim followed in the present study was an evaluation of the adaptive immune traits (cellular and humoral) of both males and females in iranian commercial vs azarbayijan native fowls. information obtained from cellular and humoral responses of 49 birds of arian broiler line. 79 azarbayijan native birds were made used of. total antibody titration, immunoglobulin y titration (igy), and immunogl...

background and aims: among the bacterial agents, the most common cause of diarrheal disease is entrotoxigenic escherichia coli. letal toxin b (ltb) subunit of lt toxin could induce six-month immunity. clostridium botulinum causes botulism disease. bont/a-hc toxin subunit could induce two years immunity against this disease. it seems that the immunogenicity potency of these two subunits may infl...

We measured hepatitis C virus antibody titers in 13 patients with chronic hepatitis C to determine whether titration of hepatitis C virus antibody was useful or not, to predict and evaluate the efficacy of interferon (IFN) treatment. During administration of IFN, hepatitis C virus titers declined in all patients. Antibody titers performed before treatment as well as just at the end of treatment...

ELISA data is usually handled in the MS Excel worksheets through different methods and different formulas. Accurate and precise calculations of the data is time consuming and tedious. ELISA Analyzer was developed with a user friendly interface to streamline titration and analysis of the data obtained from ELISA and improve rapid and lucid presentation of the results. The software can also hel...

VALIDATION OF A FOOT-AND-MOUTH DISEASE ANTIBODY ELISA IN FIVE LATIN AMERICAN COUNTRIES. The work plan consisted of using a liquid phase blocking ELISA test for the detection of antibodies to foot-andmouth disease virus (FMDV) using the following categories of sera: (A) Spot test 120 non-infected/non-vaccinated bovine sera diluted 1:32; (B) Titration test: 120 bovine sera from animals vaccinated...

A method for the titration of antigens based on separation by filtration through millipore filters of the complex of antigen with fluorescein isothiocyanate-labeled antibodies from free labeled anti­ bodies and nonspecific y-globulins is described. The fluorescence of the antigen-antibody complex is measured directly on the millipore filters using the Turner fluorometer equipped with a paper ch...

Despite the central role that antibodies play in the adaptive immune system and in biotechnology, much remains unknown about the quantitative relationship between an antibody's amino acid sequence and its antigen binding affinity. Here we describe a new experimental approach, called Tite-Seq, that is capable of measuring binding titration curves and corresponding affinities for thousands of var...