نتایج جستجو برای: bar gene
تعداد نتایج: 1168959 فیلتر نتایج به سال:
In transformed tobacco (Nicotiana tabacum) plastids, we flank the marker genes with recombinase target sites to facilitate their posttransformation excision. The P1 phage loxP sites are identical 34-bp direct repeats, whereas the phiC31 phage attB/attP sites are 54- and 215-bp sequences with partial homology within the 54-bp region. Deletions in the plastid genome are known to occur by recombin...
In order to evaluate on Glutamine Syntetase gene expression as well as amount of protein, chlorophyll and oxidative index of TBARM the experiment has been carried out using Brassica napus (Falcon cv.) in research greenhouse condition. The drought stress treatments were included -0.5, -1, -2, -4 and -6 bar that by using of equivalent water (6.5, 6, 4.7, 3, 1.1 litr respectively) have been irriga...
The CRISPR/Cas9 system enables precision editing of the genome of the model plant Arabidopsis thaliana and likely of any other organism. Tools and methods for further developing and optimizing this widespread and versatile system in Arabidopsis would hence be welcomed. Here, we designed a generic vector system that can be used to clone any sgRNA sequence in a plant T-DNA vector containing an ub...
A reproducible system for the generation of fertile, transgenic maize plants has been developed. Cells from embryogenic maize suspension cultures were transformed with the bacterial gene bar using microprojectile bombardment. Transformed calli were selected from the suspension cultures using the herbicide bialaphos. Integration of bar and activity of the enzyme phosphinothricin acetyltransferas...
Females homozygous for the sex-linked dominant, Bar, occasionally give rise to wild-type reversions and to forms with more extreme eye reduction than Bar. This behavior was shown by Sturtevant' to result from unequal crossing-over. The Bar-reverted type was considered to be a deficiency for the Bar gene; while the extreme form, called Ultra-Bar or Double-Bar, was interpreted as a duplication fo...
working draft of the human genome sequence, attention is turning once again to the controversial issue of gene patenting. In response to mounting criticism about the ease with which some groups have been able to apply for and receive patents on poorly characterized human gene sequences, the US Patent and Trademark Office (USPTO) has recently released new guidelines that will affect the outcome ...
The objective of this research was to establish a chloroplast transformation technique for Platymonas (Tetraselmis) subcordiformis. Employing the gfp gene as a reporter and the bar gene as a selectable marker, transformation vectors of P. subcordiformis chloroplast were constructed with endogenous fragments rrn16S-trnI (left) and trnA-rrn23S (right) as a recombination site of the chloroplast ge...
Rapeseed (Brassica napus L.) is the third most important oil crop in global productions. One of the major limiting factors for oilseed rape production is lepidopteran pests of the Brassicaceae family. Transgenic plants expressing Bacillus thuringiensis (Bt) genes are powerful tools in the integrated pest management of crop plants. In the present study, we used a synthetic Bt insecticidal crysta...
Instability of transgene expression in plants is often associated with complex multicopy patterns of transgene integration at the same locus, as well as position effects due to random integration. Based on maize transposable elements Activator (Ac) and Dissociation (Ds), we developed a method to generate large numbers of transgenic barley (Hordeum vulgare var Golden Promise) plants, each carryi...
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