a stable agrobacterium-mediated transformation system was established using bialaphos as the selective agent in soybeans [glycine max (l.) merr.]. the cotyledonary node explants of the soybean cultivar ‘ny-1001’ were inoculated with the agrobacterium tumefaciens strain eha105, harboring the vector pcambia3301 containing the gus gene as the reporter gene and the bar gene conferring bialaphos res...

Drought stress is one of the serious problems that restricted agronomic plant production worldwide. In molecular level, the harmful effect of drought stress is mostly caused by producing of large amount of reactive oxygen species (ROS). Catalase and Metallothionein genes have a crucial role to mope the hydrogen peroxide (H2O2) resulting reducing oxidative damage. In this research the gene expre...

drought stress is one of the serious problems that restricted agronomic plant production worldwide. inmolecular level, the harmful effect of drought stress is mostly caused by producing of large amount ofreactive oxygen species (ros). catalase and metallothionein genes have a crucial role to mope the hydrogenperoxide (h2o2) resulting reducing oxidative damage. in this research the gene expressi...

The effect of anti-sense RNA on the expression of the bialaphos resistance (bar) gene which encodes phosphinothricin acetyl transferase (PAT), was analysed in tobacco. Transient expression studies revealed that an anti-bar RNA with sequence complementarity to the complete bar coding region, inhibits PAT synthesis. To quantify the phenomenon, SR1 tobacco cells were transformed twice to introduce...

Phosphinothricin (PPT) is the active component of a family of environmentally safe, nonselective herbicides. Resistance to PPT in transgenic crops has been reported by nuclear expression of a bar transgene encoding phosphinothricin acetyltransferase, a detoxifying enzyme. We report here expression of a bacterial bar gene (b-bar1) in tobacco (Nicotiana tabacum cv Petit Havana) plastids that conf...

Genotype specificity is a big problem lagging the development of efficient hexaploid wheat transformation system. Increasingly, the biosecurity of genetically modified organisms is garnering public attention, so the generation of marker-free transgenic plants is very important to the eventual potential commercial release of transgenic wheat. In this study, 15 commercial Chinese hexaploid wheat ...

Two groups of linear gene constructs (gus and bar, and 1Ax1 and bar) lacking vector backbone sequences were independently transferred into the elite wheat (Triticum aestivum L.) variety EM12, and genetically stable transgenic plants with low copy number transgene integration were recovered. Co-transformation experiments were carried out in parallel using either circular whole plasmid(s) or line...

C. elegans is extensively used to study the Wnt-pathway and most of the core-signalling components are known. Four β-catenins are important gene expression regulators in Wnt-signalling. One of these, bar-1, is part of the canonical Wnt-pathway. Together with Wnt effector pop-1, bar-1 forms a transcription activation complex which regulates the transcription of downstream genes. The effects of b...

The invention relates to a DNA fragment containing a determined gene, the expression of which inhibits the antibiotic and herbicidal effects of Bialaphos and related products. It also relates to recombinant vectors, containing such DNA fragment, which enable this protective gene to be introduced and expressed into cells and plant cells. The claims of this patent are directed to vectors carrying...