PURPOSE To evaluate the use of Bowman's layer (BL) vertical topographic thickness maps in diagnosing keratoconus (KC). DESIGN Prospective, case control, interventional case series. PARTICIPANTS A total of 42 eyes: 22 eyes of 15 normal subjects and 20 eyes of 15 patients with KC. INTERVENTION Bowman's layer 2-dimensional 9-mm vertical topographic thickness maps were created using custom-ma...

The lateral nucleus (LA) is the input station of the amygdala for information about conditioned stimuli (CSs), whereas the medial sector of the central nucleus (CeM) is the output region that contributes most amygdala projections to brainstem fear effectors. However, there are no direct links between LA and CeM. As the main target of LA and with its strong projection to CeM, the basomedial amyg...

Malpighian tubules of the mealworm Tenebrio molitor were isolated for intracellular measurement of basolateral (V(bl)) and, indirectly, apical (V(ap)) membrane potentials. In control Ringer (50 mmol l(-1) K(+), 140 mmol l(-1) Na(+)), V(bl) was 24 mV, cell negative, and V(ap) was 48 mV, cell negative with reference to the lumen. Ion substitution experiments involving K(+) and Na(+) indicated tha...

The neuroepithelial basal lamina (BL) appears to be crucial in controlling cell-cell interactions during the early histogenesis of the nervous system. In this investigation we examined the changes in the BL in a neurological mutant of the rat previously characterized as having BL anomalies which progress to aqueductal stenosis and prenatal hydrocephalus. Embryos were obtained from matings of ra...

We have studied a transplantable carcinoma of the rat pancreas [Reddy, J. K. & Rao, M. S. (1977( Science 198, 78-80] that is composed of cytologically differentiated acinar cells that have lost their epithelial orientation and do not form acini. Light microscopy shows, however, consistent palisading, reorientation, and polarization of these cells in areas of contact with the vasculature. Electr...

p53 and PCNA expression in keratocystic odontogenic tumors compared with selected odontogenic cysts Summary: The aim of this study was to evaluate p53 and PCNA expression in different odontogenic lesions regarding their different clinical behaviors. Slices prepared from 94 paraffin-embedded tissue blocks (25 radicular cysts (RC), 23 dentigerous cysts (DC), 23 keratocystic odontogenic tumors (KC...

In the presence of 6 mmol l-1 Ba2+, known to block the K+ channels in the basal membrane, a rise in bath [K+] ([K+]bl) induced an increase in intracellular K+ concentration ([K+]i) similar in amount and in time course to that obtained in the absence of Ba2+. The presence of active and passive (other than through K+ channels) K+ uptake mechanisms across the basal membrane was investigated in dif...

A new closed advancing-layer method for generating high-aspectratio elements in the boundary-layer (BL) region is presented. This approach utilizes a recent changing topology moving mesh strategy for deforming the volume mesh as the BL is inflated. It handles very efficiently BL front collision and produces a natural smooth anisotropic blending between colliding layers. Moreover, it provides a ...

In the study of central nervous system morphogenesis, the identification of new molecular markers allows us to identify domains along the antero-posterior and dorso-ventral (DV) axes. In the past years, the alar and basal plates of the midbrain have been divided into different domains. The precise location of the alar-basal boundary is still under discussion. We have identified Barhl1, Nhlh1 an...

p53 and pcna expression in keratocystic odontogenic tumors compared with selected odontogenic cysts summary: the aim of this study was to evaluate p53 and pcna expression in different odontogenic lesions regarding their different clinical behaviors. slices prepared from 94 paraffin-embedded tissue blocks (25 radicular cysts (rc), 23 dentigerous cysts (dc), 23 keratocystic odontogenic tumors (kc...