Selecting stably expressed reference genes which are not affected by physiological or pathophysiological conditions is crucial for reliable quantification in gene expression studies. This study examined the stability of a panel twelve tissues from female mouse reproductive axis and uterus. Gene studies were carried out using reverse transcriptase quantitative polymerase chain reaction (RT-qPCR)...

The present experiment is an effort to find a stable reference gene in Camellia sinensis and Camellia assamica under different biotic and abiotic stresses. This study evaluate the variation in gene expression across tea leaf tissues in nine experiments. The suitability of 18S rRNA, 26S rRNA, rubiscobis-phosphatase oxygenase (RuBP) and Camellia tubulin (CaT) as reference genes were validated by ...

Real-time quantitative PCR (RT-qPCR) has been widely applied in uncovering disease mechanisms and screening potential biomarkers. Internal reference gene selection determines the accuracy reproducibility of data analyses. The aim this study was to identify optimal genes for relative analysis RT-qPCR fourteen NF1 related cell lines, including non-tumor, benign malignant Schwann lines. expression...

Iris domestica is a plant of the Iridaceae family and drought-tolerant, but its drought-resistance mechanism not yet clear. Analysing gene expression changes I. by qRT-PCR an important mean to understand drought resistance characteristics. Nevertheless, lack reference genes greatly hinders investigation research on adaptation at molecular genetic levels. In this study, we assessed stability 11 ...

Quantitative RT-PCR is an important technique for assessing gene expression. However, a proper normalization of reference genes prior to expression analyses of target genes is necessary. The best normalizer is that gene which remains stable in all samples from different treatments. The aim of this study was to identify stable reference genes for normalization of target genes in muscle tissue fr...

Potato (Solanum tuberosum) yield has increased dramatically over the last 50 years and this has been achieved by a combination of improved agronomy and biotechnology efforts. Gene studies are taking place to improve new qualities and develop new cultivars. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) is a bench-marking analytical tool for gene expression analysis, but ...

Selection of reference genes has become an integral step in any real time quantitative PCR (RT-qPCR) based expression studies. The importance of this study stems from the fact that riverine buffaloes are major dairy species of Indian sub-continent and the information generated here will be of great interest to the investigators engaged in functional genomic studies of this important livestock s...

Investigation of gene expression using real-time PCR (qRT-PCR) requires normalization with genes that are continuously expressed (reference genes; RGs). For accurate measurements, it is exceedingly important that RG expression is invariant under the investigated experimental conditions. It has recently become evident that RG expression may vary considerably under different culture conditions, w...