Background: To further minimize transmission risk of blood-borne viral and prion diseases, manufacturers of therapeutic animalor human-derived proteins have implemented improved in-process pathogen-removal procedures. Objectives: To evaluate removal of transmissible spongiform encephalopathy agents (prions) and viruses using Omega 100K VR membrane filtration system (Pall Filtron, Northborough, ...

enrichment of cell suspension with germ cells prior to injection into recipient seminiferous tubules is of importance in spermatogonial stem cells (sscs) transplantation. knock-out serum replacement (ksr) has been reported to enhance the proliferation of murine sscs and human embryonic stem cells. the aim of the present study was to investigate the effect of ksr versus fetal bovine serum (fbs) ...

conclusions the newly developed simple indirect elisa showed high sensitivity and specificity with respect to vnt. developing such a simple, sensitive, and specific elisa which is much less expensive than the available commercial elisa kits can improve the detection of bvdv infections, help to eliminate the disease from herds, and decrease economic losses caused by this disease. background bovi...

background: the aim of the study was to provide a point of reference to study the neospora caninum infections in china. methods: genome dna was extracted from the brains of aborted fetuses and specific pcr was performed with n. caninum nc5-targeted specific primers. fetal bovine brain tissues were homogenized and continuously cultured in vero cells with double antibodies. the medium was replace...

  Background: Ligament reconstruction is a common procedure in orthopedic surgery. Although several popular techniques are currently in use, new methods are proposed for secure fixation of the tendon graft into the bone tunnel. Purposes: We sought to introduce our new technique of Bashti bone plug for fixation of soft tissue graft in anterior cruciate ligament (ACL) reconstruction and to compar...