introduction: the tumor suppressor p53 protein can induce apoptosis in some cellular contexts. among the apoptosis-inducing genes, p53 has received the most attention for cancer gene therapy. in this study, the role of dendrosome and lipofectin mediated normal cdna of tp53 into molt-4, ccrf-cem(t-iymphoma) and k562 cell lines was assessed. material and methods: at first, ccrf-cem, mol t-4 and k...

An autocrine (noninterleukin 2) growth factor, which we term leukemia derived growth factor (LDGF), has previously been found in the culture supernatant of the human malignant T-lymphoid cell line MOLT-4f. We now show that two other human malignant T-lymphoid cell lines, CCRF-CEM and CCRF-HSB-2 also produce such a factor. All three factors, i.e., the LDGF from MOLT-4f, CCRF-CEM, and CCRF-HSB-2 ...

Background: One of the major paths for drug development is the study of bioactivities of natural products. Therefore, the aim of this study was to compare the cytotoxic effects of aqueous extract of whole Cuscuta chinensis Lam., which is a traditional medicinal herb commonly used in Iran and other oriental countries, on the human caucasian acute lymphoblastic leukemia (CCRF-CEM) and another hum...

the aim of this study was to examine the effect of crude bile on the human hepg2 and ccrf-cem cell lines. cells were exposed to different dilutions of bile. antiproliferative effects were determined by the cytotoxic mtt assay. cells undergoing apoptosis were identified by terminal deoxynucleotidyl transferase dutp nick end labeling (tunel) assay. bile administration resulted in dose-dependent c...

The aim of this study was to examine the effect of crude bile on the human HepG2 and CCRF-CEM cell lines. Cells were exposed to different dilutions of bile. Antiproliferative effects were determined by the cytotoxic MTT assay. Cells undergoing apoptosis were identified by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Bile administration resulted in dose-dependent c...

A convenient, low-cost, and highly sensitive fluorescent aptasensor for detection of leukemia has been developed based on graphene oxide-aptamer complex (GO-apt). Graphene oxide (GO) can absorb carboxyfluorescein-labeled Sgc8 aptamer (FAM-apt) by π-π stacking and quench the fluorescence through fluorescence resonance energy transfer (FRET). In the absence of Sgc8 target cell CCRF-CEM, the fluor...

Two human T-lymphoblastoid cell lines, CCRF/CEM and Molt 4, produced beta interferon (IFN-beta) upon infection with Sendai virus. Molt 4, but not CCRF/CEM, spontaneously produced up to 300 U of IFN-gamma per ml, apparently not contaminated with IFN-alpha or -beta. Phytohemagglutinin, a T-cell mitogen, did not stimulate IFN production in these lines. A third T-lymphoblastoid line, CCRF/HSB2, pro...

Three methotrexate (MTX)-resistant cell lines and their MTX-sensitive counterparts have been used to examine 2,4-diamino-6-(2,5-dimethoxybenzyl)-5-methyl-pyrido[2,3-d]pyrimidine (BW301U), a novel lipophilic antifolate, and compare its cytotoxicity with MTX and metoprine. Collateral sensitivity for both BW301U and metoprine was observed in CCRF-CEM/MTX R-cells, where MTX resistance appeared to b...

BACKGROUND: Epidemiological studies have linked the consumption of chlorinated surface waters to an increased risk of two major causes of human mortality, colorectal and bladder cancer. Trihalomethanes (THMs) are by-products formed when chlorine is used to disinfect drinking water. The purpose of this study was to examine the ability of the THMs, trichloromethane (TCM), bromodichloromethane (BD...

Human leukemia cells are being regularly grown intraperitoneally in the intact newborn Syrian hamster as 3 serially transplantable sublines of lymphosarcoma. These sublines, established from suspension cultures of human leukemic lymphoblasts (CCRF-CEM cells) by the implantation of large numbers of cells, exhibit similar transplantation and growth characteristics, and are presently in their llth...