background: in this study, we examined a new two step induction protocol for improving the differentiation of human umbilical cord blood-derived mesenchymal stem cells into neural progenitor cells. materials and methods: human umbilical cord blood-derived mesenchymal stem cells were first cultured in dulbecco’s modified eagle medium supplemented with 10% fetal bovine serum in a humidified incub...

BACKGROUND The aim of this study was the ex vivo expansion of Umbilical Cord Blood hematopoietic stem cells on biocompatible nanofiber scaffolds. MATERIALS AND METHODS CD133+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system by means of monocolonal antibody CD133 (microbeads); subsequently, flowcytometry method was done to assess the ...

To evaluate the effect of CD133(+) cells (endothelial progenitor cells) on the hypoxia-induced suppression of axonal growth of cortical neurons and the destruction of blood vessels (endothelial cells), we used anterograde axonal tracing and immunofluorescence in organ co-cultures of the cortex and the spinal cord from 3-day-old neonatal rats. CD133(+) cells prepared from human umbilical cord bl...

A significant number of hematopoietic stem/progenitor cells (HSPC) in human umbilical cord blood could serve as a reservoir for the placental vasculature, yet, their morphological and functional features are not completely understood. Here, we describe the characterization of purified CD133(+) progenitor cells from umbilical cord blood, a subset of CD34(+) hematopoietic progenitors that were gr...

objective: the peroxisome proliferator-activated receptors (ppars) are a group of nuclear receptor proteins whose functions as transcription factors regulate gene expressions. ppars play essential roles in the regulation of cellular differentiation, development, and metabolism (carbohydrate, lipid, protein), and tumorigenesis of higher organisms. this study attempts to determine the effect of b...

This study reports the treatment effect of CD133 stem cell transplantation using a corpus cavernosum angiographic surgical technique. We recruited 17 volunteers with Erectile Dysfunction (ED). The umbilical cord and peripheral blood stem cells were simultaneously isolated and collected. The peripheral blood stem cells were collected from the patient’s biological progeny by mobilization with gra...

Submit Manuscript | http://medcraveonline.com Abbreviations: UCB-SCs: Umbilical Cord Blood-Derived Stem Cells; HSCs: Hematopoietic Stem Cells; MSCs: Mesenchymal Stem Cells; UCB-MSCs: Umbilical Cord Blood-derived Mesenchymal Stem Cells; UCB-MSCs: Umbilical Cord Blood-derived MSCs; hUCB-MSCs: Human Umbilical Cord Blood-derived MSCs; GvHD: Graft-versus-Host Disease; HIV: Human Immunodeficiency Virus.

Background and Objective: Umbilical Cord blood (UCB) hematopoietic stem cell (HSC) transplantation is a therapeutic approach for the treatment of malignant and non-malignant hematologic disorders due to ease of collection, lack of risk for donors and lower levels of infection. Moreover, it is considered a good alternative for bone marrow HSC transplantation. The main limitation of their use is ...

OBJECTIVE Endothelial progenitor cells (EPC) can contribute to vascular repair and targeted tumour therapy. Little is known about generating EPC from human umbilical cord blood. We therefore compared methods for purification of EPC from human umbilical cord blood. METHODS Mononuclear cells were isolated from human umbilical cord blood by density gradient centrifugation and used either unselec...