in this study calf thymus chromatin was fractionated into active (s1 and s2) and inactive (p 2) chromatin. then the interaction of an anthracyc1ine antibiotic, adriamycin, with these fractions was investigated employing absorption and difference uv nis spectroscopy and sds and agarose gel electrophoreses. the results suggest that the binding of adriamycin to the s 1 fraction is slight but s2 an...

The standard semen analysis is the first line and the most popular laboratory test in the diagnosis of male fertility. It evaluates sperm concentration, motility, morphology and their vitality. However, it is well-known that normal results of semen analysis can not exclude men from the causes of couples' infertility. One of the most important parameters of sperm in its fertilizing potential is ...

Background: Osteopontin plays a crucial role in fertilization and subsequent embryonic development. Methods: The fresh semen samples were collected from crossbred Jersey Haryana breeding bulls. proteins the seminal plasma sperm membrane precipitated analysed by SDS-PAGE followed western blotting. Based on concentration of osteopontin plasma, bulls grouped as high (≥20 pg/ml), medium (10-20 pg/m...

background: current microscopic experimental methods cannot diagnose dna damages present in spermatozoa .therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. as reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. also, chromomycin a3 is used for evaluation of the ...

Sperm chromatin integrity has been being recognized as an important factor in male fertility. During normal fertilization, high quality sperm with intact chromatin are selected through natural selection in journey from vagina to fallopian tube. However, using Assisted Reproductive Techniques, particularly ICSI, the natural selection is bypassed. Therefore sperm with DNA breakage have the opport...

We reviewed the banded karyotypes of 24 human neuroblastomas and cell lines to identify any consistent chromosomal abnormalities. Six of the 10 primary tumors and one of the 14 cell lines were studied at this institution. Of the 24 neuroblastomas karyotyped, 20 were near-diploid, one was near-triploid, and 3 were near-tetraploid. One primary tumor had a diploid karyotype without numerical or st...

Background: Prolonged alcohol abuse in men can cause testosterone deficiency and shrinkage of the testicles (testicular atrophy). This can lead to impotence, sterility and ‘feminisation’. When alcohol is broken down in the body, it may also interfere with normal sperm structure and movement. This study was designed to determine the effects of administration of alcohol on sperm quality, sperm ma...

Background: Current microscopic experimental methods cannot diagnose DNA damages present in spermatozoa .Therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. As reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. Also, chromomycin A3 is used for evaluation of the ...

Background: Acyclovir is asynthetic purin nucleoside analog,which has been reported to be very effective against the treatment of herpes simplex and varicella zoster infections. Acyclovir is known to be toxic to gonads, but available literature fails to provide the indepth analyses of damage.The aim of the present study was to evaluate the effect of Acyclovir on DNA damage and nuclear maturatio...

Pelger-Huet anomaly is a rare autosomal dominant disorder characterized by pince-nez appearance of the nuclei of the neutrophils. This is caused by failure of nuclear segmentation due to genetic defect in the LBR (laminin B-receptor) on the chromosome 1q41-43. Heterozygotes have dumbbell-shaped or hyposegmented neutrophils with coarse chromatin and normal granules. Homozygotes have neutrophils ...