The purification of 45 KDa EMBP and the production of monospecific anti-serum is described. 45 KDa EMBP was purified by relatively simple methods using ion exchange HPLC (TSK-GEL DEAE-5PW column) and size exclusion HPLC (TSK-GEL G3000SW column). The results clearly demonstrated the speed and simplicity of the method using these columns, compared to previously-described methods for purification ...

abstract plant nonspecific lipid transfer proteins (nsltps) are divided into nsltp1 and nsltp2. the existence of an internal hydrophobic cavity, is a typical characteristic of nsltps that serves as the binding site for lipid substrates. in this communication a simple, rapid and low-cost alternative method was developed for purification of nsltp2 from rice paddy. after extracting, final supernat...

Background Aggregation of therapeutic antibody is generally occurred in its manufacturing process, and should be suppressed and removed because its potential risk for unexpected immune response [1,2]. Protein A affinity chromatography is the first purification step in the monoclonal antibody manufacturing. Although the affinity purification is a powerful technique, high affinity between protein...

essential oil of the orange peel which was extracted by hydrodistillation method showed 50 different mono- and sesquiterpenes on gc-ms analysis, in which limonene had the highest percentage (92.4%). due to various important uses of the compound, a method was developed for isolation and purification of limonene. in this method, for extra purification, a fractional distillation on vigreux column ...

A method has been developed to determine the presence of aflatoxin B1 in the urine of animals (including humans) by utilizing commercial immunochemical kits that can be used in the field. Urine is treated with diatomaceous earth and filtered to clarify the sample; 2-3 ppb aflatoxin B1, corresponding to about 300 ppb in the ingested feed/food, can be detected in the filtered urine without furthe...

The Ca2+- and phospholipid-dependent protein kinase (protein kinase C) has been purified to electrophoretic homogeneity from mouse brain cytosol. [20-3H]phorbol 12,13-dibutyrate binding activity was found to coelute quantitatively with the protein kinase activity throughout the purification procedure. The crude extract was first run over a DE52 column. Fractions containing peak activities were ...

The optimum parameters of extraction for glycoprotein from seahorse were examined and determined by Box-Behnken combined with ultrasonic extraction technology. Column chromatography of glycoprotein was used for further purification. The optimal extraction conditions of seahorse glycoprotein were extracting time 4.3 h, salt concentration 0.08 mol/L, extracting temperature 73°C, raw material, and...

BACKGROUND Streptokinase is a potent activator of plasminogen to plasmin, the enzyme that can solubilize the fibrin network in blood clots. Streptokinase is currently used in clinical medicine as a thrombolytic agent. It is naturally secreted by β-hemolytic streptococci. METHODS To reach an efficient method of purification, an immunoaffinity chromatography method was developed that could puri...

The purification and biochemical characterization of protein substrates of the ubiquitin-dependent pathway of proteolysis is made difficult in part by the low steady state levels of ubiquitin-protein conjugates. We report here on the use of a polyhistidine-tagged ubiquitin molecule (HisUb) for the purification of ubiquitin-protein conjugates by metal chelate chromatography. When Escherichia col...